| IED ID | IndEnz0002002515 |
| Enzyme Type ID | protease002515 |
| Protein Name |
ATP-dependent protease subunit HslV EC 3.4.25.2 |
| Gene Name | hslV HI_0496 |
| Organism | Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pasteurellales Pasteurellaceae Haemophilus Haemophilus influenzae Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) |
| Enzyme Sequence | MTTIVSVRRNGQVVVGGDGQVSLGNTVMKGNARKVRRLYNGKVLAGFAGGTADAFTLFELFERKLEMHQGHLLKSAVELAKDWRTDRALRKLEAMLIVADEKESLIITGIGDVVQPEEDQILAIGSGGNYALSAARALVENTELSAHEIVEKSLRIAGDICVFTNTNFTIEELPN |
| Enzyme Length | 175 |
| Uniprot Accession Number | P43772 |
| Absorption | |
| Active Site | ACT_SITE 2 |
| Activity Regulation | ACTIVITY REGULATION: Allosterically activated by HslU binding. {ECO:0000255|HAMAP-Rule:MF_00248, ECO:0000269|PubMed:12054822}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP-dependent cleavage of peptide bonds with broad specificity.; EC=3.4.25.2; Evidence={ECO:0000255|HAMAP-Rule:MF_00248}; |
| DNA Binding | |
| EC Number | 3.4.25.2 |
| Enzyme Function | FUNCTION: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (11); Chain (1); Helix (5); Initiator methionine (1); Metal binding (3); Turn (3) |
| Keywords | 3D-structure;Allosteric enzyme;Cytoplasm;Hydrolase;Metal-binding;Protease;Reference proteome;Sodium;Threonine protease |
| Interact With | P43773 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00248}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (6) |
| Cross Reference PDB | 1G3I; 1G3K; 1JJW; 1KYI; 1OFH; 1OFI; |
| Mapped Pubmed ID | 11709174; |
| Motif | |
| Gene Encoded By | |
| Mass | 19,012 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.3 uM for Arc-H(6)-SulA(CT) {ECO:0000269|PubMed:15037245}; Note=Arc-H(6)-SulA(CT) is a construct consisting of the Arc repressor protein fused to 6 His residues and the 11 carboxy terminal residues of SulA.; |
| Metal Binding | METAL 158; /note=Sodium; via carbonyl oxygen; METAL 161; /note=Sodium; via carbonyl oxygen; METAL 164; /note=Sodium; via carbonyl oxygen |
| Rhea ID | |
| Cross Reference Brenda | 3.4.25.2; |