| IED ID | IndEnz0002002543 |
| Enzyme Type ID | protease002543 |
| Protein Name |
Methionine aminopeptidase MAP MetAP EC 3.4.11.18 Peptidase M |
| Gene Name | map MTH_1296 |
| Organism | Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) |
| Taxonomic Lineage | cellular organisms Archaea Euryarchaeota Methanomada group Methanobacteria Methanobacteriales Methanobacteriaceae Methanothermobacter Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum) Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) |
| Enzyme Sequence | MIKMIESYLKAGKIVSKVRREAAGIIRDGLPIIELVNYVEDRIIEEGGRPAFPCNVSVNEVTAHYTSPPGDDSVIGDGDLVKLDLGAHVDGFIADTAITVPVGDVDDKCHQMMDAAREALENAISTIRAGVEVGEIGRVIEETIHSHGMNPVSNLTGHSMERWILHSGLSIPNINERNTHQLEEGDVLAIEPFATDGVGLVTDMPQTYIFRFLRERPLRLVHARRVLGKIREEYHALPFAQRWLEEYFDAKRLNASMRMLIQSRAIYPYHVLREKSGAMVAQWEHTVIVEEDGCTVITE |
| Enzyme Length | 299 |
| Uniprot Accession Number | O27355 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 64; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_01975; BINDING 166; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_01975 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.; EC=3.4.11.18; Evidence={ECO:0000255|HAMAP-Rule:MF_01975}; |
| DNA Binding | |
| EC Number | 3.4.11.18 |
| Enzyme Function | FUNCTION: Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). {ECO:0000255|HAMAP-Rule:MF_01975}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Binding site (2); Chain (1); Metal binding (7) |
| Keywords | Aminopeptidase;Hydrolase;Metal-binding;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 33,431 |
| Kinetics | |
| Metal Binding | METAL 84; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01975; METAL 95; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01975; METAL 95; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_01975; METAL 158; /note=Divalent metal cation 2; catalytic; via tele nitrogen; /evidence=ECO:0000255|HAMAP-Rule:MF_01975; METAL 191; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_01975; METAL 284; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01975; METAL 284; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_01975 |
| Rhea ID | |
| Cross Reference Brenda |