| IED ID | IndEnz0002002585 |
| Enzyme Type ID | protease002585 |
| Protein Name |
Glycyl-glycine endopeptidase LytM EC 3.4.24.75 Autolysin LytM |
| Gene Name | lytM NWMN_0210 |
| Organism | Staphylococcus aureus (strain Newman) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus Staphylococcus aureus Staphylococcus aureus (strain Newman) |
| Enzyme Sequence | MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQDVSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANANGSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGNGKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMPENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGDQIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR |
| Enzyme Length | 316 |
| Uniprot Accession Number | A0A0H3K6J4 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.; EC=3.4.24.75; Evidence={ECO:0000305|PubMed:24434550}; |
| DNA Binding | |
| EC Number | 3.4.24.75 |
| Enzyme Function | FUNCTION: Peptidoglycan hydrolase (autolysin) specifically acting on polyglycine interpeptide bridges of the cell wall peptidoglycan (By similarity). Releases SpA, an immunologically active peptide, from the cell wall (PubMed:24434550). {ECO:0000250|UniProtKB:O33599, ECO:0000269|PubMed:24434550}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Erroneous initiation (1); Metal binding (4); Region (1); Signal peptide (1) |
| Keywords | Cell wall biogenesis/degradation;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Virulence;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:O33599}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 34,317 |
| Kinetics | |
| Metal Binding | METAL 117; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O33599; METAL 210; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O33599; METAL 214; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O33599; METAL 293; /note=Zinc; /evidence=ECO:0000250|UniProtKB:O33599 |
| Rhea ID | |
| Cross Reference Brenda |