| IED ID | IndEnz0002002653 |
| Enzyme Type ID | protease002653 |
| Protein Name |
Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe EC 2.4.1.222 O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| Gene Name | lfng |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MLKTYRGKVVVSLAGATVTCLGFLLFLSQHQRIQADGMQNESEVGLRSLQSLGDSETDDGAQPEQNAKKGFSAYFSKLTRSRREADKPSEAPGAATDAPPAEDISADDIFIAVKTTKKFHRSRLDLLLDTWISRNMRQTYIFTDGEDEELKKKIGSHAINTNCSAAHSRQALSCKMAVEYDKFIESGKKWFCHVDDDNYVNTKTLVKLLSNYPHTQDMYIGKPSLDRPIEATERLGDNKMRPVNFWFATGGAGFCISRGLALKMSPWASGGHFMNTAEKIRLPDDCTIGYIIESVLGVSLTRSSLFHSHLENLQQVSKSEVHKQITLSYGMFENKRNIINMKGAFSVEEDPSRFKSVHCLLYPDTPWCPPQVAY |
| Enzyme Length | 374 |
| Uniprot Accession Number | Q8JHF2 |
| Absorption | |
| Active Site | ACT_SITE 285; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | BINDING 123; /note=Substrate; /evidence=ECO:0000250; BINDING 196; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.; EC=2.4.1.222; |
| DNA Binding | |
| EC Number | 2.4.1.222 |
| Enzyme Function | FUNCTION: Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Involved in the correct formation of boundaries in the somites and hindbrain (By similarity). Required for Delta-Notch-mediated induction of hypochord cells at the lateral borders of the midline precursor domain (PubMed:11429294, PubMed:14518000). {ECO:0000250|UniProtKB:O09010, ECO:0000269|PubMed:11429294, ECO:0000269|PubMed:14518000}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (2); Chain (1); Disulfide bond (3); Glycosylation (2); Metal binding (2); Region (1); Sequence conflict (6); Site (1); Topological domain (2); Transmembrane (1) |
| Keywords | Developmental protein;Differentiation;Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Metal-binding;Neurogenesis;Notch signaling pathway;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: A soluble form may be derived from the membrane form by proteolytic processing. {ECO:0000305}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 19553285; 23344707; |
| Motif | |
| Gene Encoded By | |
| Mass | 41,882 |
| Kinetics | |
| Metal Binding | METAL 197; /note=Manganese; /evidence=ECO:0000250; METAL 309; /note=Manganese; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |