| IED ID | IndEnz0002002738 |
| Enzyme Type ID | protease002738 |
| Protein Name |
Metalloendoproteinase 2-MMP At2-MMP EC 3.4.24.- |
| Gene Name | 2MMP MMP At1g70170 F20P5.11 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MRFCVFGFLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYGANPNFNGTTSPPSTTKHQRDTGGFSAAWRIDGSSRSTIVSLLLSTVGLVLWFLP |
| Enzyme Length | 378 |
| Uniprot Accession Number | O04529 |
| Absorption | |
| Active Site | ACT_SITE 281; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | ACTIVITY REGULATION: Repressed by acetohydroxamic acid (AHA). {ECO:0000269|PubMed:24156403}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Matrix metalloproteinases (MMPs) or matrixins may play a role in the degradation and remodeling of the extracellular matrix (ECM) during development or in response to stresses (By similarity). Required for plant growth, morphogenesis, and development with particular relevance for flowering and senescence (PubMed:11726650). Active on McaPLGLDpaAR-NH(2) (QF24) and myelin basic protein (MBP) and, to some extent, on beta-casein (PubMed:24156403). {ECO:0000250|UniProtKB:O23507, ECO:0000269|PubMed:11726650, ECO:0000269|PubMed:24156403}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 45 degrees Celsius. {ECO:0000269|PubMed:24156403}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7-8. {ECO:0000269|PubMed:24156403}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Glycosylation (9); Lipidation (1); Metal binding (4); Motif (1); Propeptide (2); Sequence conflict (1); Signal peptide (1) |
| Keywords | Cell membrane;Developmental protein;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | INDUCTION: Induced in seedling rosette leaves by methyl jasmonate (MeJA) and cadmium (Cd). Induced in seedling roots by salt stress (NaCl). Inhibited in leaves and inflorescences of adult plants by exposure to cadmium. {ECO:0000269|PubMed:11726650}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Lipid-anchor, GPI-anchor {ECO:0000255}; Extracellular side {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12068095; 12609040; 12615916; 12805588; 14576160; 14671020; 15235117; 18775970; 21775155; 22937923; 23858430; 28832648; |
| Motif | MOTIF 118..125; /note=Cysteine switch; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 42,006 |
| Kinetics | |
| Metal Binding | METAL 120; /note=Zinc; in inhibited form; /evidence=ECO:0000250; METAL 280; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 284; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 290; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |