| IED ID | IndEnz0002002740 |
| Enzyme Type ID | protease002740 |
| Protein Name |
Apical membrane antigen 1 TgAMA-1 TgAMA1 Cleaved into: Apical membrane antigen 1, soluble form |
| Gene Name | AMA1 TGVEG_255260 |
| Organism | Toxoplasma gondii (strain ATCC 50861 / VEG) |
| Taxonomic Lineage | cellular organisms Eukaryota Sar Alveolata Apicomplexa Conoidasida Coccidia Eucoccidiorida Eimeriorina Sarcocystidae Toxoplasma Toxoplasma gondii Toxoplasma gondii type III Toxoplasma gondii (strain ATCC 50861 / VEG) |
| Enzyme Sequence | MICSIMGGLRSLRAARPYSHQSNTETKHMGLVGVASLLVLVADCTIFASGLSSSTRSRESQTLSASTSGNPFQANVEMKTFMERFNLTHHHQSGIYVDLGQDKEVDGTLYREPAGLCPIWGKHIELQQPDRPPYRNNFLEDVPTEKEYKQSGNPLPGGFNLNFVTPSGQRISPFPMELLEKNSNIKASTDLGRCAEFAFKTVAMDKNNKATKYRYPFVYDSKKRLCHILYVSMQLMEGKKYCSVKGEPPDLTWYCFKPRKSVTENHHLIYGSAYVGENPDAFISKCPNQALRGYRFGVWKKGRCLDYTELTDTVIERVESKAQCWVKTFENDGVASDQPHTYPLTSQASWNDWWPLHQSDQPHSGGVGRNYGFYYVDTTGEGKCALSDQVPDCLVSDSAAVSYTAAGSLSEETPNFIIPSNPSVTPPTPETALQCTADKFPDSFGACDVQACKRQKTSCVGGQIQSTSVDCTADEQNECGSNTALIAGLAVGGVLLLALLGGGCYFAKRLDRNKGVQAAHHEHEFQSDRGARKKRPSDLMQEAEPSFWDEAEENIEQDGETHVMVEGDY |
| Enzyme Length | 569 |
| Uniprot Accession Number | B6KAM0 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Essential microneme protein that plays an important role in host cell invasion. Part of the moving junction (MJ) complex, a ringlike structure formed between the plasma membranes of the apical tip of the parasite and the target host cell. During invasion, the MJ migrates from the anterior to the posterior of the parasite, leading to internalization of the parasite into a parasitophorous vacuole (PV). {ECO:0000269|PubMed:11083833, ECO:0000269|PubMed:11087913, ECO:0000269|PubMed:16000372, ECO:0000269|PubMed:16102004, ECO:0000269|PubMed:16244709, ECO:0000269|PubMed:19247437, ECO:0000269|PubMed:20421941, ECO:0000269|PubMed:21205639, ECO:0000269|PubMed:22523242}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (26); Chain (2); Compositional bias (1); Disulfide bond (8); Glycosylation (1); Helix (13); Mutagenesis (7); Propeptide (1); Region (4); Sequence conflict (2); Signal peptide (1); Site (1); Topological domain (2); Transmembrane (1); Turn (6) |
| Keywords | 3D-structure;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Membrane;Reference proteome;Secreted;Signal;Tachyzoite;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}. Secreted {ECO:0000269|PubMed:11083833, ECO:0000269|PubMed:11087913, ECO:0000269|PubMed:16102004, ECO:0000269|PubMed:16244709}. Note=Initially localizes to micronemes, specialized secretory organelles of apicomplexan parasites important for host cell invasion. Relocalizes to the surface membrane upon host invasion by tachyzoites. Found predominantly on the apical end of the parasite surface. A cleavage product of the large ectodomain is released into the medium by extracellular parasites. |
| Modified Residue | |
| Post Translational Modification | PTM: Proteolytically cleaved during invasion within its transmembrane domain, releasing a soluble form from the tachyzoite surface. The cytosolic tail generated by ROM4 cleavage during invasion may trigger parasite replication within the parasitophorous vacuole. {ECO:0000269|PubMed:16102004, ECO:0000269|PubMed:20421941}. |
| Signal Peptide | SIGNAL 1..48; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 2X2Z; 2Y8R; 2Y8S; 2Y8T; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 63,021 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |