| IED ID | IndEnz0002002786 |
| Enzyme Type ID | protease002786 |
| Protein Name |
Kunitz-type serine protease inhibitor SOTI Trypsin inhibitor SOTI Fragments |
| Gene Name | |
| Organism | Spinacia oleracea (Spinach) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae Caryophyllales Chenopodiaceae Chenopodioideae Anserineae Spinacia Spinacia oleracea (Spinach) |
| Enzyme Sequence | TVAACNSPKVGLDSDAELVR |
| Enzyme Length | 20 |
| Uniprot Accession Number | P86549 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Inhibitor of bovine trypsin with a Ki of 2.3 uM. Inhibits 50% of the proteolytic activity of P.rapae larvae midgut extract at a concentration of 20 ug/ml. Inhibitory activity against trypsin is reduced by 43% in the presence of the lysine modifier TBNS, but is not affected by dithiothreitol. {ECO:0000269|PubMed:19232057}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermostable when incubated at pH 8.0 at temperatures between 40 and 100 degrees Celsius. {ECO:0000269|PubMed:19232057}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Stable at pH values ranging from 3.0-11.0 at 4 degrees Celsius. {ECO:0000269|PubMed:19232057}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-adjacent residues (1); Non-terminal residue (2) |
| Keywords | Direct protein sequencing;Protease inhibitor;Serine protease inhibitor |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,045 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |