| IED ID | IndEnz0002002808 |
| Enzyme Type ID | protease002808 |
| Protein Name |
Integrin beta-3 Platelet membrane glycoprotein IIIa GPIIIa CD antigen CD61 |
| Gene Name | Itgb3 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MRAQWPGQLWAALLALGALAGVVVGESNICTTRGVNSCQQCLAVSPVCAWCSDETLSQGSPRCNLKENLLKDNCAPESIEFPVSEAQILEARPLSSKGSGSSAQITQVSPQRIALRLRPDDSKIFSLQVRQVEDYPVDIYYLMDLSFSMKDDLSSIQTLGTKLASQMRKLTSNLRIGFGAFVDKPVSPYMYISPPQAIKNPCYNMKNACLPMFGYKHVLTLTDQVSRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVLPNDGHCHIGTDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTENVVSLYQNYSELIPGTTVGVLSDDSSNVLQLIVDAYGKIRSKVELEVRDLPEELSLSFNATCLNNEVIPGLKSCVGLKIGDTVSFSIEAKVRGCPQEKEQSFTIKPVGFKDSLTVQVTFDCDCACQAFAQPSSPRCNNGNGTFECGVCRCDQGWLGSMCECSEEDYRPSQQEECSPKEGQPICSQRGECLCGQCVCHSSDFGKITGKYCECDDFSCVRYKGEMCSGHGQCNCGDCVCDSDWTGYYCNCTTRTDTCMSTNGLLCSGRGNCECGSCVCVQPGSYGDTCEKCPTCPDACSFKKECVECKKFNRGTLHEENTCSRYCRDDIEQVKELTDTGKNAVNCTYKNEDDCVVRFQYYEDTSGRAVLYVVEEPECPKGPDILVVLLSVMGAILLIGLATLLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT |
| Enzyme Length | 787 |
| Uniprot Accession Number | O54890 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surfaces. Fibrinogen binding enhances SELP expression in activated platelets (PubMed:19332769). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (By similarity). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (By similarity). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (By similarity). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (By similarity). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (By similarity). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (By similarity). ITGAV:ITGB3 binds to the Lilrb4a/Gp49b receptor and enhances the Lilrb4a-mediated inhibition of mast cell activation (PubMed:11323698). ITGAV:ITGB3 also suppresses marginal zone B cell antibody production through its interaction with Lilrb4a (PubMed:24935931). In brain, plays a role in synaptic transmission and plasticity (PubMed:29038237, PubMed:18549786). Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin (PubMed:29038237). Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (PubMed:18549786). ITGAV:ITGB3 act as a receptor for CD40LG (By similarity). {ECO:0000250|UniProtKB:P05106, ECO:0000269|PubMed:11323698, ECO:0000269|PubMed:18549786, ECO:0000269|PubMed:19332769, ECO:0000269|PubMed:24935931, ECO:0000269|PubMed:29038237}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (1); Chain (1); Disulfide bond (28); Domain (1); Glycosylation (5); Modified residue (4); Motif (1); Mutagenesis (1); Region (4); Repeat (4); Sequence conflict (2); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | 3D-structure;Cell adhesion;Cell junction;Cell membrane;Cell projection;Disulfide bond;Glycoprotein;Integrin;Membrane;Phosphoprotein;Postsynaptic cell membrane;Receptor;Reference proteome;Repeat;Signal;Synapse;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P05106}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P05106}. Cell projection, lamellipodium membrane {ECO:0000250|UniProtKB:P05106}. Cell junction, focal adhesion {ECO:0000269|PubMed:29038237}. Cell junction, synapse, postsynaptic cell membrane {ECO:0000269|PubMed:18549786}; Single-pass type I membrane protein {ECO:0000305}. Cell junction, synapse {ECO:0000269|PubMed:29038237}. |
| Modified Residue | MOD_RES 766; /note=Phosphothreonine; /evidence=ECO:0007744|PubMed:21183079; MOD_RES 772; /note=Phosphotyrosine; /evidence=ECO:0000250|UniProtKB:P05106; MOD_RES 778; /note=Phosphothreonine; /evidence=ECO:0000250|UniProtKB:P05106; MOD_RES 784; /note=Phosphotyrosine; /evidence=ECO:0000250|UniProtKB:P05106 |
| Post Translational Modification | PTM: Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. {ECO:0000250|UniProtKB:P05106}. |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 5XQ1; 6VGU; |
| Mapped Pubmed ID | 10048576; 10091056; 10337632; 10548108; 10549293; 10683372; 11071623; 11207362; 11342577; 11353389; 11438667; 11493451; 11756480; 11786903; 11875495; 12052840; 12055214; 12377945; 12393886; 12486108; 12603314; 12618529; 12629153; 12690105; 12746502; 12761858; 12842087; 12855554; 12867986; 12900455; 12904471; 12927791; 1431091; 14516789; 14524530; 14593208; 14612570; 14718670; 15007005; 15031111; 15059849; 1512266; 15131115; 15166241; 15284116; 15329828; 15336554; 15545316; 15574772; 15591537; 15654327; 15690355; 15760908; 15972454; 16014375; 16037392; 16051597; 16150802; 16216233; 16247034; 16288021; 16294265; 16304048; 16317099; 16337627; 16365395; 16391195; 16391197; 16400188; 16407244; 16467530; 16533881; 16571912; 16600665; 16602821; 16608535; 16615898; 16622802; 16670270; 16679515; 16705383; 16720835; 16790614; 16824188; 16887829; 16995821; 17003372; 17028191; 17030947; 17062734; 17075060; 17099249; 17170121; 17184791; 17261592; 17359504; 17426256; 17446174; 17485491; 17492052; 17530709; 17597067; 17606926; 17627302; 17641225; 17898081; 17925492; 17951320; 17983596; 17996853; 18082680; 18084301; 18086863; 18156175; 18165225; 18174291; 18184798; 18255021; 18256319; 18278053; 18288635; 18317590; 18326492; 18334487; 18346467; 18366072; 18369617; 18378693; 18381073; 18430780; 18456735; 18487513; 18544684; 18552165; 18579532; 18611961; 18640270; 18829523; 18840708; 18985734; 18997092; 19005179; 19017937; 19023115; 19036705; 19074851; 19075116; 19118216; 19122172; 19218546; 19255147; 19272161; 19364763; 19454719; 19487690; 19576795; 19643138; 19645751; 19695571; 19729481; 19755424; 19758485; 19805288; 19808614; 19808644; 19837659; 19933310; 19940148; 19967726; 20007808; 20016849; 20075254; 20087368; 20110424; 20151413; 20155273; 20164359; 20184965; 2018971; 20203303; 20339539; 20393597; 20404115; 20440000; 20525921; 20564195; 20647570; 20670372; 20675382; 20826760; 20887958; 20926684; 20971951; 21035850; 21051860; 21224474; 21228213; 21254450; 21267068; 21350117; 21357746; 21368290; 21406181; 21421844; 21464228; 21622854; 21636859; 21645497; 21670307; 21677750; 21700775; 21708300; 21764795; 21937717; 21945075; 21953216; 21996747; 22019589; 22027834; 22037761; 22046433; 22074485; 22096246; 22096247; 22134168; 22210777; 22232691; 22275070; 22318609; 22349694; 22350844; 22363548; 22456341; 22479530; 22479590; 22494046; 22511753; 22527796; 22615494; 22647975; 22653978; 22730538; 22748100; 22760780; 22808199; 22812543; 22895695; 22962684; 22975325; 22984613; 22996695; 23023225; 23064962; 23086489; 23118343; 23145320; 23152823; 23176290; 23213478; 23216754; 23226954; 23230271; 23325830; 23382219; 23395612; 23454767; 23465490; 23515331; 23519467; 23551977; 23553959; 23640884; 23770013; 23788140; 23789102; 23833236; 23870190; 23955631; 24006485; 24023288; 24029230; 24030382; 24083985; 24103390; 24107997; 24151012; 24162846; 24216753; 24258817; 24292710; 24294595; 24303107; 24385425; 24441829; 24496617; 24500921; 24523237; 24585775; 24611720; 24778313; 24840544; 24859337; 24884715; 24951426; 24952961; 24969775; 25024172; 25117682; 25194508; 25352128; 25363539; 25430721; 25547154; 25587038; 25614287; 25684064; 25774504; 25820496; 25911094; 25977332; 26036802; 26159543; 26185093; 26212910; 26278818; 26291522; 26352002; 26438512; 26507506; 26529254; 26556550; 26744458; 26790499; 26858344; 26953352; 26965188; 27216180; 27233450; 27264390; 27281793; 27349568; 27452457; 27576787; 27596966; 27746115; 27812539; 27851790; 27990663; 28028021; 28220032; 28410990; 28445073; 28576878; 28650456; 28673932; 28739949; 28794456; 28815933; 28846069; 28864764; 28924218; 28962574; 29087614; 29459744; 29632205; 29739775; 29794068; 29794156; 29802249; 29853537; 30022046; 30076648; 30190457; 30235285; 30333317; 30425049; 30455459; 30458180; 30462575; 30653955; 30787865; 30931941; 31022732; 31064775; 31618629; 32237906; 32268102; 32440878; 32473126; 32817592; 32882513; 32998468; 33131250; 33153107; 33288722; 33317577; 33493267; 33818281; 33880575; 33927296; 8198553; 8306881; 8512576; 8601592; 8821033; 8918453; 8940024; 8986815; 9022036; 9339637; 9420328; 9553049; 9832163; 9849968; 9916011; 9916135; |
| Motif | MOTIF 776..782; /note=LIR; /evidence=ECO:0000250|UniProtKB:P05106 |
| Gene Encoded By | |
| Mass | 86,738 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |