| IED ID | IndEnz0002002840 |
| Enzyme Type ID | protease002840 |
| Protein Name |
MPN domain-containing protein EC 3.4.-.- |
| Gene Name | Mpnd |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MAAPESLSPGATAEEAPEEDEDDAEAEDPERGTGSGGRSGSLGGSGGGTAGPGMALGGALTRRAVTLRVLLKDELLEPGEGVLSIYYLGRKFTGDLQLDGRIVWQETGQVFNSPSAWATHCKKLVNPAKKSGCGWASVKYKGQKLDKYKAAWLRRHQLHMPVATADESPTSEGEEEELLLEEEEEDVLAGVSSEDKGHRPPGKGSLEPEATPPGKRMDKVPVPIRYCMLGSRDSARNPHTLVEVTSFAAINKFQPFNVAVSSNVLFLLDFHCHLTRSEVVGYLGGRWDINNQMLTVLRAFPCRSRLGDTDTAATVEEEIYQVLFLRGLSLVGWYHSHPHSPAVPSLQDIDAQMEYQLRLQGSSNGFQPCLALLCSPYYSGNPGPESKICPFWVMPPPEQRPSDYGIPMDVEMAYVQDSFLTNDVLQEMVMLAEFYKGAPDLVKFQEAWSPEHTYLDKLKMSLASRTPKDQGMCHVLEQVCSVLKQGS |
| Enzyme Length | 487 |
| Uniprot Accession Number | Q3TV65 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: Probable protease (By similarity). Acts as a sensor of N(6)-methyladenosine methylation on DNA (m6A): recognizes and binds m6A DNA, leading to its degradation (By similarity). {ECO:0000250|UniProtKB:Q5VVJ2, ECO:0000250|UniProtKB:Q8N594}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (2); Domain (2); Erroneous initiation (1); Frameshift (1); Initiator methionine (1); Metal binding (3); Modified residue (4); Motif (1); Region (2); Sequence conflict (1) |
| Keywords | Acetylation;Hydrolase;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 2; /note=N-acetylalanine; /evidence=ECO:0000250|UniProtKB:Q8N594; MOD_RES 8; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q8N594; MOD_RES 168; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q8N594; MOD_RES 171; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q8N594 |
| Post Translational Modification | PTM: Degraded following binding to N(6)-methyladenosine methylated DNA (m6A). {ECO:0000250|UniProtKB:Q8N594}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11217851; 12466851; 14610273; 15618518; 21677750; 22988430; 26238476; |
| Motif | MOTIF 335..348; /note=JAMM motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182 |
| Gene Encoded By | |
| Mass | 53,376 |
| Kinetics | |
| Metal Binding | METAL 335; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 337; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 348; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182 |
| Rhea ID | |
| Cross Reference Brenda |