| IED ID | IndEnz0002002845 |
| Enzyme Type ID | protease002845 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase BAP1 EC 3.4.19.12 BRCA1-associated protein 1 |
| Gene Name | bap1 TEgg031c22.1 |
| Organism | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Silurana Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
| Enzyme Sequence | MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCPGPVYGFIFLFKWIEERRSRRKVSTLLDDTSVMEDEVVNNMFFAHQLIPNSCATHALLSVLLNCSGVHLGPTLSRIKEFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGISAVRTMEAFHFVSYVPIKGRLFELDGLKVYPIDHGPWAEDEEWTDKARRVIMERIGLATAGEPYHDIRFNLMAVVPDRRLKYESKLHILKMNRQTVLEALQQLIRVTQPELIQAQKPTEGQSTEETKSAALKAPVSQESHRAHHGSHRNATDVGAEPPGALIRGPVMSAYSKPNSLAQNGGTVAAPASRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRGVPPPAPDTDEEEEEETENVRRPLTPPGFKRRSSEPLPPPPGPEPGVLAEKLKETQRDLCSPLSIKTGAPTAPHSQPSPTPSNESTDTASEIGSAFNSPLRSPLRSANPTRPSSPVTLHLSKVLFGEEEPLLRLDCVRYNRAVRELGPHISTGILHLSKDGYLCPLSRLETGKVSPKGNKVEEPRESSEPDTERSRVTEVPQGEKFSPKELLALLKCVEAEISSSEACLREELEKRKKFKIDDQRRTHNYDEFICAFISMLAQEGMLASLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ |
| Enzyme Length | 685 |
| Uniprot Accession Number | Q66JB6 |
| Absorption | |
| Active Site | ACT_SITE 91; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q92560; ACT_SITE 169; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P09936 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q92560}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) (By similarity). {ECO:0000250|UniProtKB:Q92560}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (5); Motif (1); Region (5); Sequence conflict (1); Site (1) |
| Keywords | Chromatin regulator;Cytoplasm;Hydrolase;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q92560}. Nucleus {ECO:0000250|UniProtKB:Q92560}. Note=Mainly nuclear. Binds to chromatin. {ECO:0000250|UniProtKB:Q92560}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 673..678; /note=Nuclear localization signal; /evidence=ECO:0000250|UniProtKB:Q92560 |
| Gene Encoded By | |
| Mass | 76,399 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |