| IED ID | IndEnz0002002851 |
| Enzyme Type ID | protease002851 |
| Protein Name |
E3 ubiquitin-protein ligase CHIP EC 2.3.2.27 Carboxyl terminus of HSC70-interacting protein AtCHIP Plant U-box protein 61 RING-type E3 ubiquitin transferase CHIP U-box domain-containing protein 61 |
| Gene Name | CHIP PUB61 At3g07370 F21O3.8 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MVTGVASAMAERLKEDGNNCFKKERFGAAIDAYTEAIALSPNVPAYWTNRALCHMKRKDWTKVEEDCRKAIQLVHNSVKAHYMLGLALLQKKEFTNGVKELQRALDLGRCSNPTGYMVEEIWEELSKAKYMEWELVSAMRSWELNSLKETCEAALNQQRALDMSRTEESSDEAYTAHTERLKALERVFKKAAEEDKPTEVPDYLCCNITLEIFRDPVISPSGVTYERAAILEHLKKVGKFDPITREKIDPANLVPNLAIKEAVAAYLEKHVWAYKMGC |
| Enzyme Length | 278 |
| Uniprot Accession Number | Q9SRS9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:16640601, ECO:0000269|PubMed:17241447}; |
| DNA Binding | |
| EC Number | 2.3.2.27 |
| Enzyme Function | FUNCTION: Has E3 ubiquitin-protein ligase activity and may target misfolded substrates towards proteasomal degradation. Regulates the activity of some serine/threonine-protein phosphatases by E3 ubiquitin-protein ligase activity. Required for responses to biotic and abiotic stresses such as auxin, abscisic acid (ABA), low and high temperature and darkness, probably through the activation of serine/threonine-protein phosphatase and the subsequent modification of the plasma membrane composition. Regulates the chloroplastic Clp proteolytic activity in response to stresses. Ubiquitylates FtsH1, a component of the chloroplast FtsH protease, and affects protein degradation in chloroplasts. Mediates plastid precursor degradation to prevent cytosolic precursor accumulation, together with the molecular chaperone HSC70-4. Mediates ubiquitination of transit peptides and thereby led to their degradation through the ubiquitin-proteasome system. {ECO:0000269|PubMed:12805616, ECO:0000269|PubMed:16640601, ECO:0000269|PubMed:17241447, ECO:0000269|PubMed:17714429, ECO:0000269|PubMed:20028838}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Protein modification; protein ubiquitination. {ECO:0000269|PubMed:16640601, ECO:0000269|PubMed:17241447}. |
| nucleotide Binding | |
| Features | Chain (1); Coiled coil (1); Domain (1); Repeat (3) |
| Keywords | Coiled coil;Reference proteome;Repeat;TPR repeat;Transferase;Ubl conjugation pathway |
| Interact With | |
| Induction | INDUCTION: By abiotic stresses such as chilling, heat-shock and salts (selenate and NaCl). {ECO:0000269|PubMed:12805616}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18650403; 18775970; 20856808; 23878078; 24497840; 26085677; 29237060; |
| Motif | |
| Gene Encoded By | |
| Mass | 31,655 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |