| IED ID | IndEnz0002002916 |
| Enzyme Type ID | protease002916 |
| Protein Name |
Lon protease homolog 4, chloroplastic/mitochondrial AtLon4 EC 3.4.21.53 |
| Gene Name | LON4 At3g05790 F10A16.8 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MLKFLTPTAYASHHVTPATRFRSTPVKNLLFKQLTLLTGWNRSSYELGRRSFSSDLDSDTKSSTTTVSAKPHLDDCLTVIALPLPHKPLIPGFYMPIYVKDPKVLAALQESRRQQAPYAGAFLLKDDASSDSSSSSETENILEKLKGKELINRIHEVGTLAQISSIQGEQVILIGHRQLRITEMVSESEDPLTVKVDHLKDKPYDKDDDVIKATYFQVMSTLRDVLKTTSLWRDHVRTYTQACSLHIWHCLRHIGEFNYPKLADFGAGISGANKHQNQGVLEELDVHKRLELTLELVKKEVEINKIQESIAKAVEEKFSGDRRRIILKEQINAIKKELGGETDSKSALSEKFRGRIDPIKDKIPGHVLKVIEEELKKLQLLETSSSEFDVTCNYLDWLTVLPWGNFSDENFNVLRAEKILDEDHYGLSDVKERILEFIAVGGLRGTSQGKIICLSGPTGVGKTSIGRSIARALDRKFFRFSVGGLSDVAEIKGHRRTYIGAMPGKMVQCLKNVGTENPLVLIDEIDKLGVRGHHGDPASAMLELLDPEQNANFLDHYLDVPIDLSKVLFVCTANVTDTIPGPLLDRMEVITLSGYITDEKMHIARDYLEKTARRDCGIKPEQVDVSDAAFLSLIEHYCREAGVRNLQKQIEKIFRKIALKLVRKAASTEVPRISDDVTTDTEETKSLAKTDLESPETSAEGSTVLTDELATGDPTESTTEQSGEVAETVEKYMIDESNLSDYVGKPVFQEEKIYEQTPVGVVMGLAWTSMGGSTLYIETTFVEEGEGKGGLHITGRLGDVMKESAEIAHTVARRIMLEKEPENKLFANSKLHLHVPAGATPKDGPSAGCTMITSLLSLALKKPVRKDLAMTGEVTLTGRILAIGGVKEKTIAARRSQVKVIIFPEANRRDFDELARNVKEGLEVHFVDEYEQIFELAFGYDH |
| Enzyme Length | 942 |
| Uniprot Accession Number | Q9M9L7 |
| Absorption | |
| Active Site | ACT_SITE 846; /evidence=ECO:0000255|HAMAP-Rule:MF_03120; ACT_SITE 889; /evidence=ECO:0000255|HAMAP-Rule:MF_03120 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins in presence of ATP.; EC=3.4.21.53; Evidence={ECO:0000255|HAMAP-Rule:MF_03120}; |
| DNA Binding | |
| EC Number | 3.4.21.53 |
| Enzyme Function | FUNCTION: ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner. {ECO:0000255|HAMAP-Rule:MF_03120}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 456..463; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_03120 |
| Features | Active site (2); Chain (1); Compositional bias (2); Domain (2); Modified residue (1); Nucleotide binding (1); Region (1) |
| Keywords | ATP-binding;Chloroplast;DNA-binding;Hydrolase;Membrane;Mitochondrion;Nucleotide-binding;Phosphoprotein;Plastid;Protease;Reference proteome;Serine protease;Thylakoid |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000255|HAMAP-Rule:MF_03120, ECO:0000269|PubMed:17478548}. Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:17478548}; Peripheral membrane protein {ECO:0000269|PubMed:17478548}; Stromal side {ECO:0000269|PubMed:17478548}. |
| Modified Residue | MOD_RES 54; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P93655 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12837951; 16895613; 17181860; 22023720; 24646630; 24782883; 30590727; 8982462; |
| Motif | |
| Gene Encoded By | |
| Mass | 104,925 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |