| IED ID | IndEnz0002002987 |
| Enzyme Type ID | protease002987 |
| Protein Name |
Modular serine protease EC 3.4.21.- Cleaved into: Modular serine protease non-catalytic chain; Modular serine protease catalytic chain |
| Gene Name | modSP Ldlr CG31217 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MQLISFLSNPLFFCALLLKFRTIFAACDSSQFECDNGSCISQYDVCNGEKNCPDGSDETALTCVSQRQHCTKPYFQCTYGACVIGTAGCNGVNECADGSDETRLRCGNEDDIRQHDRRLQGNCKENEFKCPSGICLDKSNFLCDGKDDCADGTGFDESVELCGHMECPAYSFKCGTGGCISGSLSCNGENDCYDGSDEAPLLCNTTKKVTTPVVTETPLELLGCPLPLGDERPILTGDGSRVLTGPITRGTVRFSCKQGYVLEGEESSYCAKNKWSTSTIPKCVKYCSTAGEFDGYSTKALCTHNGQQVECRKPFHPPGTEVKFVCSTGFKTLSPLPEMRCMKGGYWNRGRQRCEQDCGQLATPIKQFSSGGYTINNTVVPWHVGLYVWHNEKDYHFQCGGSLLTPDLVITAAHCVYDEGTRLPYSYDTFRVIAAKFYRNYGETTPEEKRRDVRLIEIAPGYKGRTENYYQDLALLTLDEPFELSHVIRPICVTFASFAEKESVTDDVQGKFAGWNIENKHELQFVPAVSKSNSVCRRNLRDIQADKFCIFTQGKSLACQGDSGGGFTSELPTNAFSTWNTARHFLFGVISNAPNADQCAHSLTVMTNIQHFEDMILNAMNRSVETRS |
| Enzyme Length | 628 |
| Uniprot Accession Number | Q9VER6 |
| Absorption | |
| Active Site | ACT_SITE 414; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 472; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 563; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease that plays a key role in innate immunity by activating the Toll pathway in response to infection with Gram-positive bacteria and fungi (PubMed:19590012, PubMed:24794300). During Gram-positive infection, acts downstream of PGRP-SA and upstream of Grass and Spz, and therefore appears to function in a pathway that links detection of Gram-positive lysine-type peptidoglycans to Toll activation (PubMed:19590012). Functions in a separate pathway to the psh-mediated activation of the Toll pathway (PubMed:19590012). {ECO:0000269|PubMed:19590012, ECO:0000269|PubMed:24794300}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (2); Disulfide bond (17); Domain (7); Glycosylation (4); Signal peptide (1); Site (1) |
| Keywords | Autocatalytic cleavage;Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Protease;Reference proteome;Repeat;Secreted;Serine protease;Signal;Sushi;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19590012}. Note=Localizes at the membrane of lipid vesicles. Secreted from the fat body into the hemolymph at the surface of these lipid vesicles. {ECO:0000269|PubMed:19590012}. |
| Modified Residue | |
| Post Translational Modification | PTM: May be proteolytically cleaved via an autocatalytic mechanism. {ECO:0000269|PubMed:19590012}. |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11172718; 11733057; 12568721; 12906807; 14605208; 17188778; 17409189; 17987029; 17996400; 19914231; 20089584; 20220848; 21074052; 21209287; 21576362; 22724070; 23071443; 23519314; 23632253; 23868318; 23944235; 25082344; 25294943; 25312911; 25421701; 25901322; 26843333; 27172210; 27678375; 27815361; 28250052; 28874153; 29091025; 29707694; 30131599; 30146479; 30367934; 31018123; 31430488; 31564469; 32063902; 32188787; 32656090; 32866212; 33827210; 33914801; 33946849; 7667301; 9060438; |
| Motif | |
| Gene Encoded By | |
| Mass | 69,433 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |