| IED ID | IndEnz0002003065 |
| Enzyme Type ID | protease003065 |
| Protein Name |
Gag polyprotein Core polyprotein Cleaved into: Matrix protein p15 MA ; RNA-binding phosphoprotein p12 pp12 ; Capsid protein p30 CA ; Nucleocapsid protein p10 NC-gag |
| Gene Name | gag |
| Organism | Murine leukemia virus (strain DEF27) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Orthoretrovirinae Gammaretrovirus Murine leukemia virus Murine leukemia virus isolates Murine leukemia virus (strain DEF27) |
| Enzyme Sequence | MGQTITTPLSLTLEHWRDVQCIASNQSVDVKRRRWVTFCSVEWPSFDVGWPLDGTFNLDIILQVKSKVFCPGPHGHPDQVPYIVTWEALAYHPPPWVKPFVSPKPFPLSTLPFSPPGPSAHPPSRSDLYTALIPSIKTKPPKSRVLPTNGGPLIDLLTENPPNLGEQGPPLPKGPVKKRRPPPPRYSPPGPMVSRLRGNRDPPAADSTTSRAFPLRLGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLTTHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGNDGRPTQLPNEVNSAFPLERPDWDYTTPEGRNHLVLYRQLLLAGLQNAGRSPTNLAKVKGITQGPSESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLKSKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRAEDEQREKERDRRRHREMSKFLATVVTGQRQDRQGGERRRPQLDKDQCAYCKEKGHWAKDCPKKPRGPRGPRPQTSLLTLGD |
| Enzyme Length | 536 |
| Uniprot Accession Number | P29168 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity). {ECO:0000250}.; FUNCTION: Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex. {ECO:0000250}.; FUNCTION: Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (5); Coiled coil (1); Compositional bias (3); Initiator methionine (1); Lipidation (1); Motif (1); Region (2); Site (3); Zinc finger (1) |
| Keywords | Capsid protein;Coiled coil;Host cell membrane;Host membrane;Lipoprotein;Membrane;Metal-binding;Myristate;Phosphoprotein;RNA-binding;Viral matrix protein;Viral nucleoprotein;Virion;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Gag polyprotein]: Virion {ECO:0000250}. Host cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}.; SUBCELLULAR LOCATION: [Matrix protein p15]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Capsid protein p30]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Nucleocapsid protein p10]: Virion {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 183..186; /note=PPXY motif; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 60,891 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |