| IED ID | IndEnz0002003113 |
| Enzyme Type ID | protease003113 |
| Protein Name |
Peptidoglycan D,D-transpeptidase FtsI EC 3.4.16.4 Penicillin-binding protein 3 PBP-3 |
| Gene Name | ftsI pbpB PA4418 |
| Organism | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Enzyme Sequence | MKLNYFQGALYPWRFCVIVGLLLAMVGAIVWRIVDLHVIDHDFLKGQGDARSVRHIAIPAHRGLITDRNGEPLAVSTPVTTLWANPKELMTAKERWPQLAAALGQDTKLFADRIEQNAEREFIYLVRGLTPEQGEGVIALKVPGVYSIEEFRRFYPAGEVVAHAVGFTDVDDRGREGIELAFDEWLAGVPGKRQVLKDRRGRVIKDVQVTKNAKPGKTLALSIDLRLQYLAHRELRNALLENGAKAGSLVIMDVKTGEILAMTNQPTYNPNNRRNLQPAAMRNRAMIDVFEPGSTVKPFSMSAALASGRWKPSDIVDVYPGTLQIGRYTIRDVSRNSRQLDLTGILIKSSNVGISKIAFDIGAESIYSVMQQVGLGQDTGLGFPGERVGNLPNHRKWPKAETATLAYGYGLSVTAIQLAHAYAALANDGKSVPLSMTRVDRVPDGVQVISPEVASTVQGMLQQVVEAQGGVFRAQVPGYHAAGKSGTARKVSVGTKGYRENAYRSLFAGFAPATDPRIAMVVVIDEPSKAGYFGGLVSAPVFSKVMAGALRLMNVPPDNLPTATEQQQVNAAPAKGGRG |
| Enzyme Length | 579 |
| Uniprot Accession Number | G3XD46 |
| Absorption | |
| Active Site | ACT_SITE 294; /note=Acyl-ester intermediate; /evidence=ECO:0000255|HAMAP-Rule:MF_02080 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.; EC=3.4.16.4; Evidence={ECO:0000255|HAMAP-Rule:MF_02080}; |
| DNA Binding | |
| EC Number | 3.4.16.4 |
| Enzyme Function | FUNCTION: Catalyzes cross-linking of the peptidoglycan cell wall at the division septum (By similarity). Binds penicillin (PubMed:20580675). {ECO:0000255|HAMAP-Rule:MF_02080, ECO:0000269|PubMed:20580675}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. {ECO:0000255|HAMAP-Rule:MF_02080}. |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (21); Chain (1); Helix (25); Region (1); Transmembrane (1); Turn (6) |
| Keywords | 3D-structure;Carboxypeptidase;Cell cycle;Cell division;Cell inner membrane;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Membrane;Peptidoglycan synthesis;Protease;Reference proteome;Septation;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: Mainly produced during exponential phase of growth. {ECO:0000269|PubMed:9045804}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-Rule:MF_02080, ECO:0000269|PubMed:20580675}; Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_02080}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (40) |
| Cross Reference PDB | 3PBN; 3PBO; 3PBQ; 3PBR; 3PBS; 3PBT; 4KQO; 4KQQ; 4KQR; 6HR4; 6HR6; 6HR9; 6HZR; 6I1E; 6R3X; 6R40; 6R42; 6UN1; 6UN3; 6VJE; 6VOT; 6Y6U; 6Y6Z; 7ATM; 7ATO; 7ATW; 7ATX; 7AU0; 7AU1; 7AU8; 7AU9; 7AUB; 7AUH; 7KIT; 7KIV; 7KIW; 7ONK; 7ONX; 7ONY; 7ONZ; |
| Mapped Pubmed ID | 31301409; 31685462; 32152075; 32420736; 33593978; 34094333; 34337941; 34356681; |
| Motif | |
| Gene Encoded By | |
| Mass | 62,856 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 2.4.1.129; |