| IED ID | IndEnz0002003114 |
| Enzyme Type ID | protease003114 |
| Protein Name |
Probable peptidoglycan D,D-transpeptidase FtsI EC 3.4.16.4 Penicillin-binding protein 3 PBP-3 |
| Gene Name | ftsI CCNA_02643 |
| Organism | Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Alphaproteobacteria Caulobacterales Caulobacteraceae Caulobacter Caulobacter vibrioides (Caulobacter crescentus) Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus) |
| Enzyme Sequence | MSLSNLGPGGVHSPLWRWVVERVWRLEHAFERSRAAARPEDDTRIRIFLVMGFFGFCFVGVSLGAGWSALFSRAGQGGGYAQGVEGARGDVVDRNGKLLAVDLAHYALYVDPREVWDAKETRAALGRALPQVPAKRLDKAVFGDHRAFVLGGLTPDEKDAIFNLGLPGVTFEEQERRMYPLGPTAAHLIGFVDSGGKGLAGAERALDDPIRKAAGGEGGPAQLSIDVRVQAALEDELRKAAEEFTPKGAVGLVTNVHTGEILGMASWPDYDANKAGGATDDQRLNRAAASVYEMGSTFKAFTVAIGLDTGVATAASTFDAREPYKLGYRTIHDYHATKAVLNLVEVFQHSSNIGTAMLAERVGGQRLSQYFTNLGLTKPAKVELQESARPLTPRKWDQDTVASTSFGHGMNISPLALAQAMNALLNGGEMRPLTIRKLPPGVRPEGRRVLSEHTSAEMLKIMRANVVPGEGGSGGKADVPGLSVGGKTGTGEKYDPAIRRYNHQRQVSSFAATFPTDGPLEADRYFVLILLDEPKGNANSFGFSTGGWVAAPAAGRVIERIAPFLGVKRKTELVTIANSPKNAAPEAGL |
| Enzyme Length | 589 |
| Uniprot Accession Number | B8H0A0 |
| Absorption | |
| Active Site | ACT_SITE 296; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:P0AD68 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.; EC=3.4.16.4; Evidence={ECO:0000250|UniProtKB:P0AD68}; |
| DNA Binding | |
| EC Number | 3.4.16.4 |
| Enzyme Function | FUNCTION: Catalyzes cross-linking of the peptidoglycan cell wall at the division septum. {ECO:0000250|UniProtKB:P0AD68}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. {ECO:0000250|UniProtKB:P0AD68}. |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Transmembrane (1) |
| Keywords | Carboxypeptidase;Cell cycle;Cell division;Cell inner membrane;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Hydrolase;Membrane;Peptidoglycan synthesis;Protease;Reference proteome;Septation;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:18786147}; Single-pass membrane protein {ECO:0000269|PubMed:18786147}. Note=Localization varies during the cell cycle. At the beginning of the cell cycle, it accumulates at the new cell pole. Later during the cell cycle, polar accumulation disappears concomitantly with the re-localization to the FtsZ ring in a FtsZ-dependent manner. A portion of FtsI molecules is also diffusely dispersed around the membrane during both cell elongation and division. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 62,995 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |