| IED ID | IndEnz0002003154 |
| Enzyme Type ID | protease003154 |
| Protein Name |
Insulin-degrading enzyme-like 1, peroxisomal EC 3.4.24.- Insulysin-like 1 Peroxisomal M16 protease Zinc-metallopeptidase |
| Gene Name | PXM16 At2g41790 T11A7.11 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAVEKSNTTVGGVEILKPRTDNREYRMIVLKNLLQVLLISDPDTDKCAASMSVSVGSFSDPQGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTASEETNYHFDVNADCFDEALDRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDGWRIRQLQKHLSKEDHPYHKFSTGNMDTLHVRPQAKGVDTRSELIKFYEEHYSANIMHLVVYGKESLDKIQDLVERMFQEIQNTNKVVPRFPGQPCTADHLQILVKAIPIKQGHKLGVSWPVTPSIHHYDEAPSQYLGHLIGHEGEGSLFHALKTLGWATGLSAGEGEWTLDYSFFKVSIDLTDAGHEHMQEILGLLFNYIQLLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLVGSSLPTKFNPAIVQKVVDELSPSNFRIFWESQKFEGQTDKAEPWYNTAYSLEKITSSTIQEWVQSAPDVHLHLPAPNVFIPTDLSLKDADDKETVPVLLRKTPFSRLWYKPDTMFSKPKAYVKMDFNCPLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFELTLLGYNHKLRILLETVVGKIANFEVKPDRFAVIKETVTKEYQNYKFRQPYHQAMYYCSLILQDQTWPWTEELDVLSHLEAEDVAKFVPMLLSRTFIECYIAGNVENNEAESMVKHIEDVLFNDPKPICRPLFPSQHLTNRVVKLGEGMKYFYHQDGSNPSDENSALVHYIQVHRDDFSMNIKLQLFGLVAKQATFHQLRTVEQLGYITALAQRNDSGIYGVQFIIQSSVKGPGHIDSRVESLLKNFESKLYEMSNEDFKSNVTALIDMKLEKHKNLKEESRFYWREIQSGTLKFNRKEAEVSALKQLQKQELIDFFDEYIKVGAARKKSLSIRVYGSQHLKEMASDKDEVPSPSVEIEDIVGFRKSQPLHGSFRGCGQPKL |
| Enzyme Length | 970 |
| Uniprot Accession Number | O22941 |
| Absorption | |
| Active Site | ACT_SITE 72; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; ACT_SITE 143; /evidence=ECO:0000305 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Peptidase that might be involved in pathogen or wound response. Not required for peroxisome biogenesis, indole-3-butyric acid (IBA) metabolism, fatty acid beta-oxidation or degradation of glyoxylate cycle enzymes during seedling development. {ECO:0000269|PubMed:19748917}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (3); Sequence conflict (1) |
| Keywords | Hydrolase;Metal-binding;Metalloprotease;Peroxisome;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:15333753}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12169696; 14701923; 20405473; 28627464; 31427085; |
| Motif | |
| Gene Encoded By | |
| Mass | 110,995 |
| Kinetics | |
| Metal Binding | METAL 69; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 73; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 150; /note=Zinc; /evidence=ECO:0000305 |
| Rhea ID | |
| Cross Reference Brenda |