| IED ID | IndEnz0002003161 |
| Enzyme Type ID | protease003161 |
| Protein Name |
Signal peptidase I S SPase I EC 3.4.21.89 Leader peptidase I |
| Gene Name | sipS BSU23310 |
| Organism | Bacillus subtilis (strain 168) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168) |
| Enzyme Sequence | MKSENVSKKKSILEWAKAIVIAVVLALLIRNFIFAPYVVDGDSMYPTLHNRERVFVNMTVKYIGEFDRGDIVVLNGDDVHYVKRIIGLPGDTVEMKNDQLYINGKKVDEPYLAANKKRAKQDGFDHLTDDFGPVKVPDNKYFVMGDNRRNSMDSRNGLGLFTKKQIAGTSKFVFYPFNEMRKTN |
| Enzyme Length | 184 |
| Uniprot Accession Number | P28628 |
| Absorption | |
| Active Site | ACT_SITE 43; ACT_SITE 83 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.; EC=3.4.21.89; |
| DNA Binding | |
| EC Number | 3.4.21.89 |
| Enzyme Function | FUNCTION: Not essential for cell viability, but required for efficient secretion of many proteins. {ECO:0000269|PubMed:10982814}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Mutagenesis (36); Topological domain (2); Transmembrane (1) |
| Keywords | Cell membrane;Hydrolase;Membrane;Protease;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: Expressed at the postexponential growth phase; regulated by the DegS-DegU system. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 21,047 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.89; |