| IED ID | IndEnz0002003220 |
| Enzyme Type ID | protease003220 |
| Protein Name |
RNA-binding protein Hfq HF-1 Host factor-I protein HF-I |
| Gene Name | hfq b4172 JW4130 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVILLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE |
| Enzyme Length | 102 |
| Uniprot Accession Number | P0A6X3 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Involved in the regulation of stress responses mediated by the sigma factors RpoS, sigma-E and sigma-32 (PubMed:17158661). Binds with high specificity to tRNAs (PubMed:18230766). Binds sRNA antitoxin RalA (PubMed:24748661). In vitro, stimulates synthesis of long tails by poly(A) polymerase I (PubMed:10677490). Required for RNA phage Qbeta replication (PubMed:805130). Seems to play a role in persister cell formation; upon overexpression decreases persister cell formation while deletion increases persister formation (PubMed:19909729). {ECO:0000269|PubMed:10677490, ECO:0000269|PubMed:17158661, ECO:0000269|PubMed:18230766, ECO:0000269|PubMed:19909729, ECO:0000269|PubMed:24748661, ECO:0000269|PubMed:805130}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (4); Chain (1); Compositional bias (1); Frameshift (1); Helix (2); Initiator methionine (1); Region (1) |
| Keywords | 3D-structure;DNA-binding;Direct protein sequencing;RNA-binding;Reference proteome;Stress response |
| Interact With | P0AG30 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (17) |
| Cross Reference PDB | 1HK9; 2Y90; 2YHT; 3GIB; 3QHS; 3QO3; 3RER; 3RES; 3VU3; 4JRI; 4JRK; 4JUV; 4RCB; 4RCC; 4V2S; 5UK7; 6QLB; |
| Mapped Pubmed ID | 11804582; 11804583; 12093755; 14526009; 15690043; 16606699; 19889981; 20472786; 21543856; 21673658; 21979921; 23226421; 24223139; 24288369; 25551292; 4550762; 7984093; |
| Motif | |
| Gene Encoded By | |
| Mass | 11,166 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |