| IED ID | IndEnz0002003273 |
| Enzyme Type ID | protease003273 |
| Protein Name |
Radiation response metalloprotease IrrE EC 3.4.24.- DNA repair regulatory protein IrrE Inducer of pleiotropic protein for DNA repair |
| Gene Name | irrE pprI DR_0167 |
| Organism | Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Deinococcus-Thermus Deinococci Deinococcales Deinococcaceae Deinococcus Deinococcus radiodurans Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) |
| Enzyme Sequence | MPSANVSPPCPSGVRGGGMGPKAKAEASKPHPQIPVKLPFVTAPDALAAAKARMRDLAAAYVAALPGRDTHSLMAGVPGVDLKFMPLGWRDGAFDPEHNVILINSAARPERQRFTLAHEIGHAILLGDDDLLSDIHDAYEGERLEQVIETLCNVAAAAILMPEPVIAEMLERFGPTGRALAELAKRAEVSASSALYALTEQTPVPVIYAVCAPGKPPREQAASDEDAGPSTEKVLTVRASSSTRGVKYTLASGTPVPADHPAALALATGMEVREESYVPFRSGRKMKAEVDAYPSRGIVAVSFEFDPARLGRKDSEQADRDEPQDAAQ |
| Enzyme Length | 328 |
| Uniprot Accession Number | Q9RXY7 |
| Absorption | |
| Active Site | ACT_SITE 119; /evidence=ECO:0000250|UniProtKB:C1CZ84 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Plays a central regulatory role in DNA repair and protection pathways in response to radiation stress (PubMed:12804570, PubMed:22051194). Acts as a site-specific metalloprotease that cleaves and inactivates the repressor protein DdrO, resulting in induced expression of genes required for DNA repair and cell survival after exposure to radiation (By similarity). Regulates the expression of dozens of proteins from different pathways, including the important DNA repair proteins RecA and PprA (PubMed:12399492, PubMed:12804570, PubMed:22051194). Binds to the promoters of recA and pprA (PubMed:22051194). {ECO:0000250|UniProtKB:C1CZ84, ECO:0000269|PubMed:12399492, ECO:0000269|PubMed:12804570, ECO:0000269|PubMed:22051194}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3); Region (3) |
| Keywords | DNA-binding;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Stress response;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 34,757 |
| Kinetics | |
| Metal Binding | METAL 118; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:C1CZ84; METAL 122; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:C1CZ84; METAL 149; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:C1CZ84 |
| Rhea ID | |
| Cross Reference Brenda |