| IED ID | IndEnz0002003309 |
| Enzyme Type ID | protease003309 |
| Protein Name |
Gag polyprotein Core polyprotein Cleaved into: Matrix protein p15 MA ; RNA-binding phosphoprotein p12 pp12 ; Capsid protein p30 CA ; Nucleocapsid protein p10-Gag NC-gag |
| Gene Name | gag |
| Organism | AKV murine leukemia virus (AKR (endogenous) murine leukemia virus) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Orthoretrovirinae Gammaretrovirus Murine leukemia virus AKV murine leukemia virus (AKR (endogenous) murine leukemia virus) |
| Enzyme Sequence | MGQTVTTPLSLTLEHWEDVQRIASNQSVDVKKRRWVTFCSAEWPTFGVGWPQDGTFNLDIILQVKSKVFSPGPHGHPDQVPYIVTWEAIAYEPPPWVKPFVSPKLSPSPTAPILPSGPSTQPPPRSALYPALTPSIKPRPSKPQVLSDNGGPLIDLLSEDPPPYGGQGLSSSDGDGDREEATSTSEIPAPSPIVSRLRGKRDPPAADSTTSRAFPLRLGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLTTHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGNDGRPTQLPNEVDAAFPLERPDWDYTTQRGRNHLVLYRQLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLKSKTLGDLVREAERIFNKRETPEEREERVRRETEEKEERRRAEEEQKEKERDRRRHREMSKLLATVVSGQRQDRQGGERRRPQLDKDQCAYCKEKGHWAKDCPKKPRGPRGPRPQTSLLTLDD |
| Enzyme Length | 537 |
| Uniprot Accession Number | P03336 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: [Gag polyprotein]: Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release. {ECO:0000250|UniProtKB:P03332}.; FUNCTION: [Matrix protein p15]: Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex. {ECO:0000250|UniProtKB:P03332}.; FUNCTION: [RNA-binding phosphoprotein p12]: Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes. {ECO:0000250|UniProtKB:P03332}.; FUNCTION: [Capsid protein p30]: Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex. {ECO:0000250|UniProtKB:P03332}.; FUNCTION: [Nucleocapsid protein p10-Gag]: Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization. {ECO:0000250|UniProtKB:P03332}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (3); Chain (5); Coiled coil (1); Compositional bias (3); Helix (6); Initiator methionine (1); Lipidation (1); Modified residue (1); Motif (3); Region (4); Site (3); Turn (1); Zinc finger (1) |
| Keywords | 3D-structure;Alternative initiation;Capsid protein;Coiled coil;Host cell membrane;Host cytoplasm;Host endosome;Host membrane;Host-virus interaction;Lipoprotein;Membrane;Metal-binding;Myristate;Phosphoprotein;RNA-binding;Ubl conjugation;Viral budding;Viral budding via the host ESCRT complexes;Viral matrix protein;Viral nucleoprotein;Viral release from host cell;Virion;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Gag polyprotein]: Virion {ECO:0000250|UniProtKB:P03332}. Host cell membrane {ECO:0000250|UniProtKB:P03332}; Lipid-anchor {ECO:0000250|UniProtKB:P03332}. Host endosome, host multivesicular body {ECO:0000250|UniProtKB:P26807}.; SUBCELLULAR LOCATION: [Matrix protein p15]: Virion {ECO:0000250|UniProtKB:P03332}.; SUBCELLULAR LOCATION: [Capsid protein p30]: Virion {ECO:0000250|UniProtKB:P03332}.; SUBCELLULAR LOCATION: [Nucleocapsid protein p10-Gag]: Virion {ECO:0000250|UniProtKB:P03332}.; SUBCELLULAR LOCATION: [RNA-binding phosphoprotein p12]: Host cytoplasm {ECO:0000250|UniProtKB:P03332}. Note=Localizes to the host cytoplasm early in infection and binds to the mitotic chromosomes later on. {ECO:0000250|UniProtKB:P03332}. |
| Modified Residue | MOD_RES 191; /note=Phosphoserine; by host; /evidence=ECO:0000250|UniProtKB:P03332 |
| Post Translational Modification | PTM: [Gag polyprotein]: Ubiquitinated by ITCH. Gag can recruit the ubiquitin ligase Itch in an L domain-independent manner to facilitate virus release via a mechanism that involves Gag ubiquitination. {ECO:0000250|UniProtKB:P03332}.; PTM: [Gag polyprotein]: Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation. {ECO:0000250|UniProtKB:P03332}.; PTM: [Capsid protein p30]: Sumoylated; required for virus replication. {ECO:0000250|UniProtKB:P03332}.; PTM: RNA-binding phosphoprotein p12 is phosphorylated on serine residues. {ECO:0000250|UniProtKB:P03332}. |
| Signal Peptide | |
| Structure 3D | Electron microscopy (3); X-ray crystallography (3) |
| Cross Reference PDB | 1U7K; 2Y4Z; 3BP9; 6HWW; 6HWX; 6HWY; |
| Mapped Pubmed ID | 18222469; 21483490; 22804908; 30478053; |
| Motif | MOTIF 109..112; /note=PTAP/PSAP motif; /evidence=ECO:0000250|UniProtKB:P03332; MOTIF 128..132; /note=LYPX(n)L motif; /evidence=ECO:0000250|UniProtKB:P03332; MOTIF 161..164; /note=PPXY motif; /evidence=ECO:0000250|UniProtKB:P03332 |
| Gene Encoded By | |
| Mass | 60,558 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |