| IED ID | IndEnz0002003319 |
| Enzyme Type ID | protease003319 |
| Protein Name |
Gag polyprotein Core polyprotein Cleaved into: Matrix protein p15 MA ; RNA-binding phosphoprotein p12 pp12 ; Capsid protein p30 CA ; Nucleocapsid protein p10 NC-gag |
| Gene Name | gag |
| Organism | Moloney murine sarcoma virus (MoMSV) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Orthoretrovirinae Gammaretrovirus Moloney murine sarcoma virus (MoMSV) |
| Enzyme Sequence | MGQTVTTPLSLTLDHWKDVERLAHNQSVDVKKRRWVTFCSAEWPTFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKPPPPLLPSAPSLPLEPPLSTPPQSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTEDPPPYRDPRPPPSDRDGDSGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLRTGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWEYTTQAGRNHLVHYRQLLIAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLRNKTLGDLVREAERIFNKRETPEEREERIRREREEKEERRRTEDEQKEKERDRRRHREMSRLLATVVSGQRQDRQEGERRRSQLDCDQCTYCEEQGHWAKDCPRRPRGPRGPRPQTSLLTLDD |
| Enzyme Length | 538 |
| Uniprot Accession Number | P03334 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release (By similarity). {ECO:0000250}.; FUNCTION: Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity). {ECO:0000250}.; FUNCTION: Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex. {ECO:0000250}.; FUNCTION: Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (5); Compositional bias (3); Initiator methionine (1); Lipidation (1); Motif (3); Natural variant (1); Region (3); Site (3); Zinc finger (1) |
| Keywords | Capsid protein;Direct protein sequencing;Host cell membrane;Host membrane;Host-virus interaction;Lipoprotein;Membrane;Metal-binding;Myristate;Viral budding;Viral budding via the host ESCRT complexes;Viral matrix protein;Viral nucleoprotein;Viral release from host cell;Virion;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Gag polyprotein]: Virion {ECO:0000250}. Host cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}.; SUBCELLULAR LOCATION: [Matrix protein p15]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Capsid protein p30]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Nucleocapsid protein p10]: Virion {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 111..114; /note=PTAP/PSAP motif; MOTIF 130..134; /note=LYPX(n)L motif; MOTIF 162..165; /note=PPXY motif |
| Gene Encoded By | |
| Mass | 61,209 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |