| IED ID | IndEnz0002003458 |
| Enzyme Type ID | protease003458 |
| Protein Name |
ESX-1 secretion-associated protein EspB Antigen MTB48 |
| Gene Name | espB mtb48 Rv3881c MTV027.16c |
| Organism | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Enzyme Sequence | MTQSQTVTVDQQEILNRANEVEAPMADPPTDVPITPCELTAAKNAAQQLVLSADNMREYLAAGAKERQRLATSLRNAAKAYGEVDEEAATALDNDGEGTVQAESAGAVGGDSSAELTDTPRVATAGEPNFMDLKEAARKLETGDQGASLAHFADGWNTFNLTLQGDVKRFRGFDNWEGDAATACEASLDQQRQWILHMAKLSAAMAKQAQYVAQLHVWARREHPTYEDIVGLERLYAENPSARDQILPVYAEYQQRSEKVLTEYNNKAALEPVNPPKPPPAIKIDPPPPPQEQGLIPGFLMPPSDGSGVTPGTGMPAAPMVPPTGSPGGGLPADTAAQLTSAGREAAALSGDVAVKAASLGGGGGGGVPSAPLGSAIGGAESVRPAGAGDIAGLGQGRAGGGAALGGGGMGMPMGAAHQGQGGAKSKGSQQEDEALYTEDRAWTEAVIGNRRRQDSKESK |
| Enzyme Length | 460 |
| Uniprot Accession Number | P9WJD9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Required for host-cell death and may support an EsxA-independent virulence function (PubMed:23869560). Secreted processed form of EspB binds to phosphatidic acid and phosphatidylserine (PubMed:23869560). Inhibits IFN-gamma-induced autophagy in murine macrophages (PubMed:25641595). {ECO:0000269|PubMed:23869560, ECO:0000269|PubMed:25641595}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (2); Chain (1); Helix (9); Initiator methionine (1); Modified residue (1); Region (3); Site (2) |
| Keywords | 3D-structure;Acetylation;Direct protein sequencing;Reference proteome;Secreted;Virulence |
| Interact With | Itself |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17676952, ECO:0000269|PubMed:23869560}. Note=Secreted via the ESX-1 / type VII secretion system (T7SS) (PubMed:17676952). Secretion requires both EspK and EccCb1, but not EspA, EspC and EspD (PubMed:17676952, PubMed:23869560). {ECO:0000269|PubMed:17676952, ECO:0000269|PubMed:23869560}. |
| Modified Residue | MOD_RES 2; /note=N-acetylthreonine; /evidence=ECO:0007744|PubMed:21969609 |
| Post Translational Modification | PTM: Cleaved in the C-terminal region by MycP1 (PubMed:17676952, PubMed:20227664, PubMed:23620593, PubMed:24113528). The exact location of the cleavage sites has been the subject of conflicting reports (PubMed:20227664, PubMed:23620593). Further in vitro studies confirmed that Ala-358 is the primary cleavage site and Ala-386 is a secondary cleavage site (PubMed:24113528). Likely translocated as a full-length protein into the periplasm, where it is proteolyzed by MycP1 (PubMed:17676952, PubMed:20227664). Proteolysis may serve to limit the secretion of other ESX-1 substrates (PubMed:20227664). {ECO:0000269|PubMed:17676952, ECO:0000269|PubMed:20227664, ECO:0000269|PubMed:23620593, ECO:0000269|PubMed:24113528}. |
| Signal Peptide | |
| Structure 3D | Electron microscopy (3); X-ray crystallography (4) |
| Cross Reference PDB | 3J83; 4XWP; 4XXN; 4XXX; 4XY3; 6XZC; 7P13; |
| Mapped Pubmed ID | 32875288; 34337436; |
| Motif | |
| Gene Encoded By | |
| Mass | 47,594 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |