| IED ID | IndEnz0002003459 |
| Enzyme Type ID | protease003459 |
| Protein Name |
Serine protease EspC EC 3.4.21.- Cleaved into: Secreted autotransporter protein EspC EPEC-secreted protein C ; Autotransporter protein EspC translocator |
| Gene Name | espC E2348C_2915 |
| Organism | Escherichia coli O127:H6 (strain E2348/69 / EPEC) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli O127:H6 Escherichia coli O127:H6 (strain E2348/69 / EPEC) |
| Enzyme Sequence | MNKIYALKYCHATGGLIAVSELASRVMKKAARGSLLALFNLSLYGAFLSASQAAQLNIDNVWARDYLDLAQNKGVFKAGATNVSIQLKNGQTFNFPNVPIPDFSPASNKGATTSIGGAYSVTATHNGTTHHAISTQNWGQSSYKYIDRMTNGDFAVTRLDKFVVETTGVKNSVDFSLNSHDALERYGVEINGEKKIIGFRVGAGTTYTVQNGNTYSTGQVYNPLLLSASMFQLNWDNKRPYNNTTPFYNETTGGDSGSGFYLYDNVKKEWVMLGTLFGIASSGADVWSILNQYDENTVNGLKNKFTQKVQLNNNTMSLNSDSFTLAGNNTAVEKNNNNYKDLSFSGGGSINFDNDVNIGSGGLIFDAGHHYTVTGNNKTFKGAGLDIGDNTTVDWNVKGVVGDNLHKIGAGTLNVNVSQGNNLKTGDGLVVLNSANAFDNIYMASGHGVVKINHSAALNQNNDYRGIFFTENGGTLDLNGYDQSFNKIAATDIGALITNSAVQKAVLSVNNQSNYMYHGSVSGNTEINHQFDTQKNNSRLILDGNVDITNDINIKNSQLTMQGHATSHAVFREGGVTCMLPGVICEKDYVSGIQQQENSANKNNNTDYKTNNQVSSFEQPDWENRLFKFKTLNLINSDFIVGRNAIVVGDISANNSTLSLSGKDTKVHIDMYDGKNITGDGFGFRQDIKDGVSVSPESSSYFGNVTLNNHSLLDIGNKFTGGIEAYDSSVSVTSQNAVFDRVGSFVNSSLTLEKGAKLTAQGGIFSTGAVDVKENASLILTGTPSAQKQEYYSPVISTTEGINLGDKASLSVKNMGYLSSDIHAGTTAATINLGDGDAETDSPLFSSLMKGYNAVLSGNITGEQSTVNMNNALWYSDGNSTIGTLKSTGGRVELGGGKDFATLRVKELNANNATFLMHTNNSQADQLNVTNKLLGSNNTVLVDFLNKPASEMNVTLITAPKGSDEKTFTAGTQQIGFSNVTPVISTEKTDDATKWMLTGYQTVSDAGASKTATDFMASGYKSFLTEVNNLNKRMGDLRDTQGDAGVWARIMNGTGSADGGYSDNYTHVQIGADRKHELDGVDLFTGALLTYTDSNASSHAFSGKTKSVGGGLYASALFDSGAYFDLIGKYLHHDNQYTASFASLGTKDYSSHSWYAGAEVGYRYHLSEESWVEPQMELVYGSVSGKSFSWEDRGMALSMKDKDYNPLIGRTGVDVGRTFSGDDWKITARAGLGYQFDLLANGETVLRDASGEKRFEGEKDSRMLMNVGMNAEIKDNMRFGLELEKSAFGKYNVDNAINANFRYSF |
| Enzyme Length | 1305 |
| Uniprot Accession Number | Q9EZE7 |
| Absorption | |
| Active Site | ACT_SITE 125; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028; ACT_SITE 153; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028; ACT_SITE 256; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028 |
| Activity Regulation | ACTIVITY REGULATION: Inhibition of cytotoxic activity by phenylmethylsulfonyl fluoride. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease with enterotoxic and cytotoxic activities. Cleaves fodrin, but does not cause its redistribution within epithelial cells. The exact role of EspC in EPEC pathogenesis is still unknown. {ECO:0000269|PubMed:15155671}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (3); Domain (2); Frameshift (1); Mutagenesis (1); Sequence conflict (9); Signal peptide (1); Site (1) |
| Keywords | Cell outer membrane;Direct protein sequencing;Hydrolase;Membrane;Periplasm;Protease;Secreted;Serine protease;Signal;Transmembrane;Transmembrane beta strand;Virulence;Zymogen |
| Interact With | |
| Induction | INDUCTION: Expression is repressed by glucose. |
| Subcellular Location | SUBCELLULAR LOCATION: [Serine protease EspC]: Periplasm {ECO:0000250}.; SUBCELLULAR LOCATION: [Secreted autotransporter protein EspC]: Secreted. Cell surface.; SUBCELLULAR LOCATION: [Autotransporter protein EspC translocator]: Cell outer membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Note=The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: Cleaved to release the mature protein from the outer membrane. |
| Signal Peptide | SIGNAL 1..53; /evidence=ECO:0000269|PubMed:7644526 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 140,782 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |