| IED ID | IndEnz0002003563 |
| Enzyme Type ID | protease003563 |
| Protein Name |
Gamma-terpinene synthase, chloroplastic EC 4.2.3.114 Terpene synthase 2 TcTPS2 |
| Gene Name | TPS2 |
| Organism | Thymus caespititius (Cretan thyme) (Origanum caespititium) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Lamiales Lamiaceae Nepetoideae Mentheae Thymus Thymus caespititius (Cretan thyme) (Origanum caespititium) |
| Enzyme Sequence | MATLSMQVSILSKQVKNLNSFGMRASKLPMVARRVDVSTTRLRPICSASLQVEEETRRSGNYQASIWDNAFIQSFNTNKYRDEKHLNRKEELIAQVKVLLNTKMEAVKQLELIDDLRNLGLTYYFQDEFKKILTCIYNDHKCFKNEQVGDLYFTSLGFRLLRLHGFDVSEDVFSFFKNEDGSDFKASLGENTKDVLQLYEASFPIRVGEVTLEQARVFSTKILEKKVDEGINDEKLLAWIQHSLALPLHWRIQRLEARWFLDAYAARKDMNPLIFELGKIDFHIIQETQLEEVQEVSRWWTNSNLAEKLPFVRDRIVECYFWALGLFEPHEYGYQRKMAAIIITFVTIIDDVYDVYGTLDELQLFTDAIRKWDLESISTLPYYMQVCYLALYTYASELAYDILKDQGFNSISYLQRSWLSLVEGFFQEAKWYYAGYTPTLAEYLENAKVSISSPTIISQVYFTLPNSNEGTVVENVYGYHNMLYLSGMILRLADDLGTTQFELKRGDVQKAIQCYMKDNNATEKEGQEHVKYLLREAWKEMNTAMADPDCPLSEDLVDAAANLGRASQFIYLEGDGHGVQHSEIHNQMGGLIFEPYV |
| Enzyme Length | 597 |
| Uniprot Accession Number | R4JND9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(2E)-geranyl diphosphate = diphosphate + gamma-terpinene; Xref=Rhea:RHEA:32559, ChEBI:CHEBI:10577, ChEBI:CHEBI:33019, ChEBI:CHEBI:58057; EC=4.2.3.114; Evidence={ECO:0000269|PubMed:23624978};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32560; Evidence={ECO:0000269|PubMed:23624978}; |
| DNA Binding | |
| EC Number | 4.2.3.114 |
| Enzyme Function | FUNCTION: Involved in the biosynthesis of phenolic monoterpenes natural products thymol and carvacrol which have a broad range of biological activities acting as antimicrobial compounds, insecticides, antioxidants and pharmaceutical agents (PubMed:23624978). Monoterpene synthase which catalyzes the conversion of geranyl diphosphate (GPP) to gamma-terpinene and minor amounts of other monoterpenes (e.g. alpha-thujene, alpha-terpinene, myrcene, sabinene, (+)-R-limonene, alpha-pinene and alpha-phellandrene) (PubMed:23624978). {ECO:0000269|PubMed:23624978}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. {ECO:0000269|PubMed:23624978}. |
| nucleotide Binding | |
| Features | Chain (1); Metal binding (6); Motif (1); Region (2); Sequence conflict (3); Transit peptide (1) |
| Keywords | Chloroplast;Lyase;Magnesium;Manganese;Metal-binding;Plastid;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 350..354; /note=DDXXD motif; /evidence=ECO:0000250|UniProtKB:Q9X839 |
| Gene Encoded By | |
| Mass | 69,183 |
| Kinetics | |
| Metal Binding | METAL 350; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:A0A1C9J6A7; METAL 350; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:A0A1C9J6A7; METAL 354; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:A0A1C9J6A7; METAL 354; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:A0A1C9J6A7; METAL 494; /note=Manganese 3; /evidence=ECO:0000250|UniProtKB:A0A1C9J6A7; METAL 502; /note=Manganese 3; /evidence=ECO:0000250|UniProtKB:A0A1C9J6A7 |
| Rhea ID | RHEA:32559; RHEA:32560 |
| Cross Reference Brenda |