Detail Information for IndEnz0002003572
IED ID IndEnz0002003572
Enzyme Type ID protease003572
Protein Name Gag polyprotein
Pr71Gag

Cleaved into: Gag protein
p68Gag
; p3
p3Gag
Gene Name gag
Organism Human spumaretrovirus (SFVcpz(hu)) (Human foamy virus)
Taxonomic Lineage Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Spumaretrovirinae Spumavirus Simian foamy virus Human spumaretrovirus (SFVcpz(hu)) (Human foamy virus)
Enzyme Sequence MASGSNVEEYELDVEALVVILRDRNIPRNPLHGEVIGLRLTEGWWGQIERFQMVRLILQDDDNEPLQRPRYEVIQRAVNPHTMFMISGPLAELQLAFQDLDLPEGPLRFGPLANGHYVQGDPYSSSYRPVTMAETAQMTRDELEDVLNTQSEIEIQMINLLELYEVETRALRRQLAERSSTGQGGISPGAPRSRPPVSSFSGLPSLPSIPGIHPRAPSPPRATSTPGNIPWSLGDDSPPSSSFPGPSQPRVSFHPGNPFVEEEGHRPRSQSRERRREILPAPVPSAPPMIQYIPVPPPPPIGTVIPIQHIRSVTGEPPRNPREIPIWLGRNAPAIDGVFPVTTPDLRCRIINAILGGNIGLSLTPGDCLTWDSAVATLFIRTHGTFPMHQLGNVIKGIVDQEGVATAYTLGMMLSGQNYQLVSGIIRGYLPGQAVVTALQQRLDQEIDNQTRAETFIQHLNAVYEILGLNARGQSIRASVTPQPRPSRGRGRGQNTSRPSQGPANSGRGRQRPASGQSNRGSSTQNQNQDNLNQGGYNLRPRTYQPQRYGGGRGRRWNDNTNNQESRPSDQGSQTPRPNQAGSGVRGNQSQTPRPAAGRGGRGNHNRNQRSSGAGDSRAVNTVTQSATSSTDESSSAVTAASGGDQRD
Enzyme Length 648
Uniprot Accession Number P14349
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Involved in capsid formation and genome binding. Shortly after infection, interaction between incoming particle-associated Gag proteins and host dynein allows centrosomal targeting of the viral genome (associated to Gag), prior to nucleus translocation and integration into host genome. {ECO:0000269|PubMed:12857789, ECO:0000269|PubMed:16160174}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (12); Chain (3); Compositional bias (6); Helix (16); Motif (2); Mutagenesis (9); Region (6); Site (4); Turn (5)
Keywords 3D-structure;Capsid protein;Cytoplasmic inwards viral transport;DNA-binding;Host cytoplasm;Host nucleus;Host-virus interaction;Microtubular inwards viral transport;RNA-binding;Reference proteome;Viral budding;Viral budding via the host ESCRT complexes;Viral nucleoprotein;Viral release from host cell;Virion;Virus entry into host cell
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: [Gag protein]: Virion. Host nucleus. Host cytoplasm. Note=Nuclear at initial phase, cytoplasmic at assembly. Shortly after infection, Gag protein is targeted to centrosomes. It is then actively transported into the nucleus thanks to its nuclear localization signal. In the late phases of infection, Gag proteins assemble in the cytoplasm to form the virion's capsids.; SUBCELLULAR LOCATION: [p3]: Virion.
Modified Residue
Post Translational Modification PTM: Specific enzymatic cleavages in vivo by viral protease yield mature proteins. The protease is not cleaved off from Pol. Since cleavage efficiency is not optimal for all sites, intermediary molecules are expressed. {ECO:0000269|PubMed:10438890}.
Signal Peptide
Structure 3D NMR spectroscopy (2); X-ray crystallography (3)
Cross Reference PDB 4JMR; 4JNH; 5M1G; 5M1H; 5MLU;
Mapped Pubmed ID 23675305; 27829070; 28490494;
Motif MOTIF 284..287; /note=PTAP/PSAP motif; MOTIF 535..556; /note=Nuclear localization signal; /evidence=ECO:0000269|PubMed:8970944
Gene Encoded By
Mass 70,591
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.4.23.B11;