| IED ID | IndEnz0002003582 |
| Enzyme Type ID | protease003582 |
| Protein Name |
Trypsin inhibitor 1 JcTI-I Fragment |
| Gene Name | |
| Organism | Jatropha curcas (Barbados nut) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Malpighiales Euphorbiaceae Crotonoideae Jatropheae Jatropha Jatropha curcas (Barbados nut) |
| Enzyme Sequence | VRDICKKEAERQDLSSCENYITQRRGY |
| Enzyme Length | 27 |
| Uniprot Accession Number | C0HJF7 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Inhibits trypsin (IC(50)=1.25 uM) but not chymotrypsin or papain. Has antibacterial activity against S.enterica ATCC 10708 (MIC=5 ug/ml) and S.aureus ATCC 25923 (MIC=5 ug/ml) but not against B.subtilis ATCC 6633 or P.aeruginosa ATCC 25619. Has no hemolytic activity against human erythrocytes. Is not toxic to mice. {ECO:0000269|PubMed:24523715}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Trypsin inhibitory activity is stable after incubation at 90 degrees Celsius for up to 2 hours. {ECO:0000269|PubMed:24523715}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Trypsin inhibitory activity is stable after incubation at pH 2 to pH 10 at 60 degrees Celsius for 1 hour. {ECO:0000269|PubMed:24523715}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Antibiotic;Antimicrobial;Direct protein sequencing;Disulfide bond;Glycoprotein;Protease inhibitor;Serine protease inhibitor |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Contains disulfide bonds. {ECO:0000269|PubMed:24523715}.; PTM: Glycosylated. {ECO:0000269|PubMed:24523715}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 3,262 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |