| IED ID | IndEnz0002003599 |
| Enzyme Type ID | protease003599 |
| Protein Name |
Serine protease grass EC 3.4.21.- Gram-positive specific serine protease |
| Gene Name | grass c-SP1 CG5896 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MMIASSLAVLYGIAIVSSMGVQSARADYADDCTTPDGDQGQCMPFSSCRTIEERLTEAQKAGQKVPADYASYLQKALCGEFNGVRHFCCPSANIQHNSKVMSLFKDENFDCGNFLSQRVSNGYEVKLSSRPWMALLRYQQFGESRFLCGGAMISERYILTAAHCVHGLQNDLYEIRLGEHRISTEEDCRQQGRKKKCAPPVVNVGIEKHLIHEKYDARHIMHDIALLKLNRSVPFQKHIKPICLPITDELKEKAEQISTYFVTGWGTTENGSSSDVLLQANVPLQPRSACSQAYRRAVPLSQLCVGGGDLQDSCKGDSGGPLQAPAQYLGEYAPKMVEFGIVSQGVVTCGQISLPGLYTNVGEYVQWITDTMASNGL |
| Enzyme Length | 377 |
| Uniprot Accession Number | Q9VB68 |
| Absorption | |
| Active Site | ACT_SITE 163; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 223; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 318; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Endopeptidase (By similarity). Plays a key role in innate immunity by activating the Toll pathway in response to fungal and Gram-positive bacterial infections, presumably downstream of pattern-recognition receptors (PRR), such as PGRP-SA, GNBP1 and GNBP3, and upstream of spz processing enzyme SPE (PubMed:16631589, PubMed:18724373). {ECO:0000250|UniProtKB:Q9XXV0, ECO:0000269|PubMed:16631589, ECO:0000269|PubMed:18724373}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Beta strand (19); Chain (1); Disulfide bond (8); Domain (2); Erroneous initiation (1); Glycosylation (2); Helix (11); Metal binding (4); Region (1); Signal peptide (1); Turn (4) |
| Keywords | 3D-structure;Alternative splicing;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:21310954}. Note=Probably secreted in the hemolymph. {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Proteolytically cleaved by a tryspin-like protease which is likely to activate grass. {ECO:0000269|PubMed:21310954}. |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000269|PubMed:21310954 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 2XXL; |
| Mapped Pubmed ID | 12364793; 12568721; 15466469; 17588928; 17996400; 18776638; 19504457; 19520911; 19543372; 19590012; 19740772; 19851448; 20089584; 20220848; 21074052; 21209287; 21278706; 21576362; 22227521; 22724070; 23071443; 23632253; 23868318; 24443439; 24658702; 24928808; 25000897; 25901322; 26162375; 26453810; 27936378; 28627812; 29211760; 29265369; 29268741; 29452635; 29707694; 30146479; 30367934; 30865231; 31018123; 31722958; 32866212; 33827210; |
| Motif | |
| Gene Encoded By | |
| Mass | 41,511 |
| Kinetics | |
| Metal Binding | METAL 179; /note="Calcium"; /evidence="ECO:0000269|PubMed:21310954, ECO:0007744|PDB:2XXL"; METAL 181; /note="Calcium; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:21310954, ECO:0007744|PDB:2XXL"; METAL 184; /note="Calcium; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:21310954, ECO:0007744|PDB:2XXL"; METAL 187; /note="Calcium"; /evidence="ECO:0000269|PubMed:21310954, ECO:0007744|PDB:2XXL" |
| Rhea ID | |
| Cross Reference Brenda |