| IED ID | IndEnz0002003604 |
| Enzyme Type ID | protease003604 |
| Protein Name |
Adenosine 5'-monophosphoramidase HINT3 EC 3.9.1.- HINT-4 Histidine triad nucleotide-binding protein 3 HINT-3 |
| Gene Name | Hint3 Hint4 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MAEKQAGLVGEPDPEGSSPGTSESWNYDSNCVFCRVAAGQEPKTELFHCENEDLVCFKDIKPAALYHYLVVPKKHIGSCKDLNKDHIEMVESMVAAGKTMLERNNFTDFTDVRMGFHVPPFCSISHLHLHVIAPVKEFGFLSKLVYRQDSYWFVTVDYLLEKLRK |
| Enzyme Length | 165 |
| Uniprot Accession Number | Q9CPS6 |
| Absorption | |
| Active Site | ACT_SITE 128; /note=Tele-AMP-histidine intermediate; /evidence=ECO:0000250|UniProtKB:Q9NQE9 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=adenosine 5'-phosphoramidate + H2O = AMP + NH4(+); Xref=Rhea:RHEA:67916, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:57890, ChEBI:CHEBI:456215; Evidence={ECO:0000250|UniProtKB:Q9NQE9}; |
| DNA Binding | |
| EC Number | 3.9.1.- |
| Enzyme Function | FUNCTION: Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2 (By similarity). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase (By similarity). {ECO:0000250|UniProtKB:Q9NQE9}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 59..60; /note=AMP; /evidence=ECO:0000250|UniProtKB:P49773; NP_BIND 128..130; /note=AMP; /evidence=ECO:0000250|UniProtKB:P49773 |
| Features | Active site (1); Chain (1); Domain (1); Initiator methionine (1); Modified residue (1); Motif (1); Nucleotide binding (2); Region (1) |
| Keywords | Acetylation;Cytoplasm;Hydrolase;Nucleotide-binding;Nucleus;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9NQE9}. Nucleus {ECO:0000250|UniProtKB:Q9NQE9}. |
| Modified Residue | MOD_RES 2; /note=N-acetylalanine; /evidence=ECO:0000250|UniProtKB:Q9NQE9 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11217851; 12466851; 21267068; 21677750; 28609438; |
| Motif | MOTIF 126..130; /note=Histidine triad motif |
| Gene Encoded By | |
| Mass | 18,788 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:67916 |
| Cross Reference Brenda |