| IED ID | IndEnz0002003693 |
| Enzyme Type ID | protease003693 |
| Protein Name |
Apoptotic protease-activating factor 1 APAF-1 |
| Gene Name | Apaf1 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MDAKARNCLLQHREALEKDIKTSYIMDHMISNGVLSVIEEEKVKSQATQYQRAAALIKMILNKDNCAYISFYNALLHEGYKDLAALLQSGLPLVSSSSGKDTDGGITSFVRTVLCEGGVPQRPVIFVTRKKLVHAIQQKLWKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSLLEGCFSGGVHWVSIGKQDKSGLLMKLQNLCMRLDQEESFSQRLPLNIEEAKDRLRVLMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKHVVPVESGLGREKGLEILSLFVNMKKEDLPAEAHSIIKECKGSPLVVSLIGALLRDFPNRWAYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCVLWDLETEEVEDILQEFVNKSLLFCNRNGKSFCYYLHDLQVDFLTEKNRSQLQDLHRKMVTQFQRYYQPHTLSPDQEDCMYWYNFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVAYRHILDEKDCAVCENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYRQAKLQAKQEGDTGRLYLEWINKKTIKNLSRLVVRPHTDAVYHACFSQDGQRIASCGADKTLQVFKAETGEKLLDIKAHEDEVLCCAFSSDDSYIATCSADKKVKIWDSATGKLVHTYDEHSEQVNCCHFTNKSNHLLLATGSNDFFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDELLASCSADGTLRLWDVRSANERKSINVKRFFLSSEDPPEDVEVIVKCCSWSADGDKIIVAAKNKVLLFDIHTSGLLAEIHTGHHSTIQYCDFSPYDHLAVIALSQYCVELWNIDSRLKVADCRGHLSWVHGVMFSPDGSSFLTASDDQTIRVWETKKVCKNSAIVLKQEIDVVFQENETMVLAVDNIRGLQLIAGKTGQIDYLPEAQVSCCCLSPHLEYVAFGDEDGAIKIIELPNNRVFSSGVGHKKAVRHIQFTADGKTLISSSEDSVIQVWNWQTGDYVFLQAHQETVKDFRLLQDSRLLSWSFDGTVKVWNVITGRIERDFTCHQGTVLSCAISSDATKFSSTSADKTAKIWSFDLLSPLHELKGHNGCVRCSAFSLDGILLATGDDNGEIRIWNVSDGQLLHSCAPISVEEGTATHGGWVTDVCFSPDSKTLVSAGGYLKWWNVATGDSSQTFYTNGTNLKKIHVSPDFRTYVTVDNLGILYILQVLE |
| Enzyme Length | 1249 |
| Uniprot Accession Number | O88879 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 265; /note=ATP |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 154..161; /note=ATP; /evidence=ECO:0000255 |
| Features | Alternative sequence (1); Beta strand (65); Binding site (1); Chain (1); Domain (2); Helix (29); Mutagenesis (1); Nucleotide binding (1); Region (1); Repeat (15); Sequence conflict (3); Turn (16) |
| Keywords | 3D-structure;ATP-binding;Alternative splicing;Apoptosis;Calcium;Cytoplasm;Nucleotide-binding;Reference proteome;Repeat;WD repeat |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 3SFZ; 3SHF; |
| Mapped Pubmed ID | 10101687; 10342853; 10403638; 10421635; 10452843; 10508592; 10656767; 10830166; 11067867; 11217851; 11279485; 11423996; 11504943; 11566499; 11731416; 11859117; 11859416; 11919205; 12062423; 12107826; 12121437; 12147675; 12351193; 12374983; 12466851; 12520002; 12660414; 12773531; 12883670; 14561754; 14610273; 14709542; 14713951; 14747474; 15105372; 15118096; 15210730; 15257302; 15371421; 15649468; 15703386; 15776018; 15843406; 15861188; 15907471; 16021178; 16086359; 16183742; 16215690; 16291596; 16294213; 16317003; 16397188; 16407291; 16465443; 16514420; 16537572; 16595687; 16670300; 16777102; 16799012; 16917506; 16919269; 16932756; 16954146; 17079275; 17218955; 17218959; 17274640; 17380155; 17562856; 17576813; 17652622; 18032672; 18037375; 18042457; 18056406; 18093951; 18256270; 18272486; 18480964; 18483489; 18497877; 18562310; 18663378; 18951482; 19139631; 19144853; 19420217; 19675677; 19913007; 19914347; 19960021; 20349273; 20624980; 20720205; 20966962; 21221781; 21267068; 21431351; 21677750; 21984814; 22162136; 22206758; 22226830; 23197294; 23892366; 24369835; 24412373; 24416260; 24632563; 24651435; 25028717; 25285627; 25330150; 25349173; 25525875; 25940086; 25975226; 26334721; 26374523; 27001308; 27580417; 27789626; 29068314; 29596528; 30938680; 31178404; 31212066; 32979334; 33278357; 34518561; 9092658; 9216040; 9708735; 9753321; 9988219; |
| Motif | |
| Gene Encoded By | |
| Mass | 141,003 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |