| IED ID | IndEnz0002003727 |
| Enzyme Type ID | protease003727 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase MINDY-3 EC 3.4.19.12 Deubiquitinating enzyme MINDY-3 Protein CARP |
| Gene Name | mindy3 carp fam188a zgc:153892 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MSDTNKEVVDLVWGRPSGGGVPASLFRRWSQGFVFSETERSALEQFEGGPCAVIAPVQAFLLKNILFNTEGLNWKDISEEEQRTVLCSTLSEILELACLNKSQAFHLVTWPHAKTTDNSDITDSHPEPESSQPTDTPTALATEELGFERFHSVIQKRTLRTVAELKEAVLSLYDTWKNKFGVLLFLYSVILTKGIENIKNEIEDTTEPLIDPVYGHGSQSLINLLVTGHAVSNVWDGDRECSGMKLHGIYQQASVGFLTLMESLRYCKVGAFLKSPKFPIWILGSETHLSVFFTKEMALVAPESASEQARRVFQTFDPEDNGFIPDTLLEDVMKALDLVSEPDYVNLMKSKLDPEGLGIILLGQFLLEFFPDQDSVIPDSFPVYHYNGLKQSNHNEKVSYVEGTALVMGFEDPMVRTDDTPVKRCLQTKWPYIELLWTTERSPSLN |
| Enzyme Length | 446 |
| Uniprot Accession Number | A0AUR5 |
| Absorption | |
| Active Site | ACT_SITE 51; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q8N5J2; ACT_SITE 288; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q8N5J2 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q9H8M7}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins. {ECO:0000250|UniProtKB:Q9H8M7}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Region (1) |
| Keywords | Apoptosis;Hydrolase;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9H8M7}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 50,111 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |