| IED ID | IndEnz0002003747 |
| Enzyme Type ID | protease003747 |
| Protein Name |
NLR family CARD domain-containing protein 4 CARD, LRR, and NACHT-containing protein CED-4-like protein Clan Caspase recruitment domain-containing protein 12 Ice protease-activating factor Ipaf |
| Gene Name | NLRC4 CARD12 CLAN CLAN1 IPAF UNQ6189/PRO20215 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MNFIKDNSRALIQRMGMTVIKQITDDLFVWNVLNREEVNIICCEKVEQDAARGIIHMILKKGSESCNLFLKSLKEWNYPLFQDLNGQSLFHQTSEGDLDDLAQDLKDLYHTPSFLNFYPLGEDIDIIFNLKSTFTEPVLWRKDQHHHRVEQLTLNGLLQALQSPCIIEGESGKGKSTLLQRIAMLWGSGKCKALTKFKFVFFLRLSRAQGGLFETLCDQLLDIPGTIRKQTFMAMLLKLRQRVLFLLDGYNEFKPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRQFGALTAEVGDMTEDSAQALIREVLIKELAEGLLLQIQKSRCLRNLMKTPLFVVITCAIQMGESEFHSHTQTTLFHTFYDLLIQKNKHKHKGVAASDFIRSLDHCGDLALEGVFSHKFDFELQDVSSVNEDVLLTTGLLCKYTAQRFKPKYKFFHKSFQEYTAGRRLSSLLTSHEPEEVTKGNGYLQKMVSISDITSTYSSLLRYTCGSSVEATRAVMKHLAAVYQHGCLLGLSIAKRPLWRQESLQSVKNTTEQEILKAININSFVECGIHLYQESTSKSALSQEFEAFFQGKSLYINSGNIPDYLFDFFEHLPNCASALDFIKLDFYGGAMASWEKAAEDTGGIHMEEAPETYIPSRAVSLFFNWKQEFRTLEVTLRDFSKLNKQDIRYLGKIFSSATSLRLQIKRCAGVAGSLSLVLSTCKNIYSLMVEASPLTIEDERHITSVTNLKTLSIHDLQNQRLPGGLTDSLGNLKNLTKLIMDNIKMNEEDAIKLAEGLKNLKKMCLFHLTHLSDIGEGMDYIVKSLSSEPCDLEEIQLVSCCLSANAVKILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDRMNVLEQLTALMLPWGCDVQGSLSSLLKHLEEVPQLVKLGLKNWRLTDTEIRILGAFFGKNPLKNFQQLNLAGNRVSSDGWLAFMGVFENLKQLVFFDFSTKEFLPDPALVRKLSQVLSKLTFLQEARLVGWQFDDDDLSVITGAFKLVTA |
| Enzyme Length | 1024 |
| Uniprot Accession Number | Q9NPP4 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity). {ECO:0000250|UniProtKB:Q3UP24, ECO:0000269|PubMed:15107016}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 169..176; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00136 |
| Features | Alternative sequence (5); Chain (1); Domain (2); Helix (7); Modified residue (1); Natural variant (3); Nucleotide binding (1); Region (2); Repeat (12); Sequence conflict (6) |
| Keywords | 3D-structure;ATP-binding;Alternative splicing;Apoptosis;Cytoplasm;Disease variant;Immunity;Inflammasome;Inflammatory response;Innate immunity;Leucine-rich repeat;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat |
| Interact With | P29466; Itself; Q48824 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11390368}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q3UP24}. Inflammasome {ECO:0000269|PubMed:15107016, ECO:0000269|PubMed:33420028}. |
| Modified Residue | MOD_RES 533; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q3UP24 |
| Post Translational Modification | PTM: Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro. {ECO:0000250|UniProtKB:Q3UP24}. |
| Signal Peptide | |
| Structure 3D | Electron microscopy (3) |
| Cross Reference PDB | 6K8J; 6MKS; 6N1I; |
| Mapped Pubmed ID | 11278253; 12545166; 12646168; 15190255; 15528373; 15580302; 16817903; 16984919; 17284521; 17418785; 17620097; 17690885; 17935074; 18453601; 20303873; 20349122; 21918512; 22227487; 22547706; 23012363; 23215645; 24054992; 24516390; 25342284; 25605939; 25811392; 26258904; 26269955; 26362438; 26972847; 27175981; 27203668; 27708283; 27750030; 27974463; 28011505; 28992059; 29797527; 30279182; 30368901; 30385506; 30658261; 30802690; 30824190; 31133717; 31244842; 31511355; 31597739; 31751447; 32397236; 32531834; 32802832; 32833985; 33846244; 33848452; 34099337; |
| Motif | |
| Gene Encoded By | |
| Mass | 116,159 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |