| IED ID | IndEnz0002003768 |
| Enzyme Type ID | protease003768 |
| Protein Name |
Mitochondrial-processing peptidase subunit beta EC 3.4.24.64 Beta-MPP |
| Gene Name | PMPCB |
| Organism | Bos taurus (Bovine) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine) |
| Enzyme Sequence | MAAAAVARAVLFSAARRRLCGFTERLLIGGAAGRSLYFGGNRLRSTQAAAQVVLNVPETRVTCLENGLRVASEDSGLATCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSTLGEAEIERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSINRKDLVDYITTHYKGPRIVLAAAGGVSHDELLELAKFHFGESLSTHKGEIPALPPCKFTGSEIRVRDDKMPLAHLAVAVEAVGWAHPDTICLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCHSFQSFNTSYTDTGLWGIYMVCEPATVADMLHVVQKEWMRLCTSVTESEVARAKNLLKTNMLLQLDGSTPICEDIGRQMLCYNRRIPIPELEARIDAVNAEIIREVCTKYIYDKSPAVAAVGPIEQLPDFNQICSNMRWLHD |
| Enzyme Length | 490 |
| Uniprot Accession Number | Q3SZ71 |
| Absorption | |
| Active Site | ACT_SITE 105; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Activity Regulation | ACTIVITY REGULATION: Binding to PMPCA is required for catalytic activity. {ECO:0000250|UniProtKB:P10507}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.; EC=3.4.24.64; Evidence={ECO:0000250|UniProtKB:Q03346}; |
| DNA Binding | |
| EC Number | 3.4.24.64 |
| Enzyme Function | FUNCTION: Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins (By similarity). Preferentially, cleaves after an arginine at position P2 (By similarity). Required for PINK1 turnover by coupling PINK1 mitochondrial import and cleavage, which results in subsequent PINK1 proteolysis (By similarity). {ECO:0000250|UniProtKB:O75439, ECO:0000250|UniProtKB:Q03346}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3); Site (2); Transit peptide (1) |
| Keywords | Hydrolase;Metal-binding;Metalloprotease;Mitochondrion;Protease;Reference proteome;Transit peptide;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250|UniProtKB:O75439}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 54,237 |
| Kinetics | |
| Metal Binding | METAL 102; /note=Zinc; via tele nitrogen; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 106; /note=Zinc; via tele nitrogen; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 182; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Rhea ID | |
| Cross Reference Brenda |