IED ID | IndEnz0002003778 |
Enzyme Type ID | protease003778 |
Protein Name |
Coagulase/fibrinolysin EC 3.4.23.48 Plasminogen activator |
Gene Name | pla YPPCP1.07 YP_pPCP08 |
Organism | Yersinia pestis |
Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Yersiniaceae Yersinia Yersinia pseudotuberculosis complex Yersinia pestis |
Enzyme Sequence | MKKSSIVATIITILSGSANAASSQLIPNISPDSFTVAASTGMLSGKSHEMLYDAETGRKISQLDWKIKNVAILKGDISWDPYSFLTLNARGWTSLASGSGNMDDYDWMNENQSEWTDHSSHPATNVNHANEYDLNVKGWLLQDENYKAGITAGYQETRFSWTATGGSYSYNNGAYTGNFPKGVRVIGYNQRFSMPYIGLAGQYRINDFELNALFKFSDWVRAHDNDEHYMRDLTFREKTSGSRYYGTVINAGYYVTPNAKVFAEFTYSKYDEGKGGTQTIDKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF |
Enzyme Length | 312 |
Uniprot Accession Number | P17811 |
Absorption | |
Active Site | ACT_SITE 104; /evidence=ECO:0000250; ACT_SITE 106; /evidence=ECO:0000250; ACT_SITE 226; /evidence=ECO:0000250; ACT_SITE 228; /evidence=ECO:0000250 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Converts human Glu-plasminogen to plasmin by cleaving the 560-Arg-|-Val-561 peptide bond that is also hydrolyzed by the mammalian u-plasminogen activator and t-plasminogen activator. Also cleaves arginyl bonds in other proteins.; EC=3.4.23.48; |
DNA Binding | |
EC Number | 3.4.23.48 |
Enzyme Function | FUNCTION: Seems to play an essential role in plague transmission by mediating flea blockage in a temperature-dependent fashion. Fibrinolytic activity prevails at 37 degrees Celsius whereas coagulase expression predominates at lower temperatures (<30 degrees Celsius). Activates plasminogen by cleaving it. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (4); Beta strand (14); Chain (1); Helix (3); Sequence conflict (1); Signal peptide (1); Turn (3) |
Keywords | 3D-structure;Aspartyl protease;Blood coagulation;Cell outer membrane;Fibrinolysis;Hemostasis;Hydrolase;Membrane;Plasmid;Protease;Reference proteome;Signal;Transmembrane;Transmembrane beta strand |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
Structure 3D | X-ray crystallography (4) |
Cross Reference PDB | 2X4M; 2X55; 2X56; 4DCB; |
Mapped Pubmed ID | 20637417; 22645135; |
Motif | |
Gene Encoded By | Plasmid pPCP1; Plasmid pKYP1 |
Mass | 34,611 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda | 3.4.23.48;3.4.23.49; |