Detail Information for IndEnz0002003778
IED ID IndEnz0002003778
Enzyme Type ID protease003778
Protein Name Coagulase/fibrinolysin
EC 3.4.23.48
Plasminogen activator
Gene Name pla YPPCP1.07 YP_pPCP08
Organism Yersinia pestis
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Yersiniaceae Yersinia Yersinia pseudotuberculosis complex Yersinia pestis
Enzyme Sequence MKKSSIVATIITILSGSANAASSQLIPNISPDSFTVAASTGMLSGKSHEMLYDAETGRKISQLDWKIKNVAILKGDISWDPYSFLTLNARGWTSLASGSGNMDDYDWMNENQSEWTDHSSHPATNVNHANEYDLNVKGWLLQDENYKAGITAGYQETRFSWTATGGSYSYNNGAYTGNFPKGVRVIGYNQRFSMPYIGLAGQYRINDFELNALFKFSDWVRAHDNDEHYMRDLTFREKTSGSRYYGTVINAGYYVTPNAKVFAEFTYSKYDEGKGGTQTIDKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF
Enzyme Length 312
Uniprot Accession Number P17811
Absorption
Active Site ACT_SITE 104; /evidence=ECO:0000250; ACT_SITE 106; /evidence=ECO:0000250; ACT_SITE 226; /evidence=ECO:0000250; ACT_SITE 228; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Converts human Glu-plasminogen to plasmin by cleaving the 560-Arg-|-Val-561 peptide bond that is also hydrolyzed by the mammalian u-plasminogen activator and t-plasminogen activator. Also cleaves arginyl bonds in other proteins.; EC=3.4.23.48;
DNA Binding
EC Number 3.4.23.48
Enzyme Function FUNCTION: Seems to play an essential role in plague transmission by mediating flea blockage in a temperature-dependent fashion. Fibrinolytic activity prevails at 37 degrees Celsius whereas coagulase expression predominates at lower temperatures (<30 degrees Celsius). Activates plasminogen by cleaving it.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (4); Beta strand (14); Chain (1); Helix (3); Sequence conflict (1); Signal peptide (1); Turn (3)
Keywords 3D-structure;Aspartyl protease;Blood coagulation;Cell outer membrane;Fibrinolysis;Hemostasis;Hydrolase;Membrane;Plasmid;Protease;Reference proteome;Signal;Transmembrane;Transmembrane beta strand
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000255
Structure 3D X-ray crystallography (4)
Cross Reference PDB 2X4M; 2X55; 2X56; 4DCB;
Mapped Pubmed ID 20637417; 22645135;
Motif
Gene Encoded By Plasmid pPCP1; Plasmid pKYP1
Mass 34,611
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.4.23.48;3.4.23.49;