| IED ID | IndEnz0002003797 |
| Enzyme Type ID | protease003797 |
| Protein Name |
Fibrinolytic enzyme 2 NJF-2 EC 3.4.21.- Fragments |
| Gene Name | |
| Organism | Hediste japonica (Polychaete worm) (Neanthes japonica) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Spiralia Lophotrochozoa Annelida Polychaeta (polychaetes) Errantia Phyllodocida Nereididae (sandworms) Hediste Hediste japonica (Polychaete worm) (Neanthes japonica) |
| Enzyme Sequence | ISGTSMSCPHVAGRAYVLDTSLRVYLLDTGLR |
| Enzyme Length | 32 |
| Uniprot Accession Number | P86876 |
| Absorption | |
| Active Site | ACT_SITE 5; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10082 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by PMSF. Not inhibited by benzamidine, aprotinin, SBTI, EDTA, EGTA, 2-mercaptoethanol, iodoacetic acid or pepstatin A. {ECO:0000269|Ref.1}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease. Has fibrinolytic and fibrinogenolytic but no plasminogenolytic activity. Cleaves after Arg and Lys residues. Cleaves fibrinogen alpha chain, beta chain and gamma chain in that order. {ECO:0000269|Ref.1}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 degrees Celsius. Active from 30 to 70 degrees Celsius. {ECO:0000269|Ref.1}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 10. Active from pH 7 to 11. {ECO:0000269|Ref.1}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Non-adjacent residues (2); Non-terminal residue (2) |
| Keywords | Blood coagulation;Direct protein sequencing;Fibrinogenolytic toxin;Fibrinolysis;Hemostasis;Hemostasis impairing toxin;Hydrolase;Protease;Serine protease;Toxin |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 3,453 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |