| IED ID | IndEnz0002003859 |
| Enzyme Type ID | protease003859 |
| Protein Name |
Lon protease homolog, mitochondrial EC 3.4.21.53 |
| Gene Name | Lon CG8798 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MLARAIRVRPMMRGIASSSVWNRNRPIQSSLMQYCRDRSLRLQRLHGANLMVQRFYSRKRDDSNGDIIMGPDLMSDQDTHLPATVAVPDVWPHVPLLAMRKNPLFPRFMKIVEVSNPIIMDLLRRKVKLNQPYVGVFLKKTDGEEELITNLNDVYNLGTFAQIQELQDLGDKLRMVVVAHRRIRITGQVVEDVPPPKPVKMTTLHYPLFNIKLQIPAEDQSTDQADAAPIKSRSDPARKPRGRIPRSRTGKSRESAAAEELIQNQTLEPPLKSGKVESSSLPKPPTEEKIVEPETGAKENVNQSAPSAQPVLIVEVENVKQPIYKQTEEVKALTQEIIKTLRDIITMNPLYRESLQQMLHQNQRVVDNPIYLCDLGASLSAGEPAELQKILEETDIPERLQLALTLLKKELELSRLQQKIGREVEEKVKQQHRKYILQEQLKVIKKELGIEKDDKDAIGEKYREKLKDKVVPEAIMTVIDEELTKLNFLESHSSEFNVTRNYLDWLTSLPWGVISTENLCLEKATETLNDDHYGMEDIKKRILEFIAVSSLKGSTQGKILCFHGPPGVGKTSIAKSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKLIQCLKKTKIENPLVLIDEVDKIGKGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSRVLFICTANVIDTIPEPLRDRMELIEMSGYVAEEKIAIARQYLMPQAMKDCGLTDKHINISEDALNMLIRSYCRESGVRNLQKHIEKVIRKVAFRVVKKEGEHFPVNADNLTTFLGKQIFSSDRMYATTPVGVVMGLAWTAMGGSSLYIETSRRHIRQGAKTDPNTVAGSLHITGNLGDVMKESAQIALTVARNFLYSLEPNNLFLEQEHIHLHVPEGATPKDGPSAGITIITALVSLATGKPVRQDIAMTGEVSLKGKVLPVGGIKEKTIAARRSGVNCLILPVDNKKDFEELPTYITDGLEVHFATTYEDVYKIAFTDVTETTTNNVEEQEPLQKLSSAAAAKSETWPYS |
| Enzyme Length | 1024 |
| Uniprot Accession Number | Q7KUT2 |
| Absorption | |
| Active Site | ACT_SITE 898; /evidence=ECO:0000255|HAMAP-Rule:MF_03120; ACT_SITE 941; /evidence=ECO:0000255|HAMAP-Rule:MF_03120 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins in presence of ATP.; EC=3.4.21.53; Evidence={ECO:0000255|HAMAP-Rule:MF_03120}; |
| DNA Binding | |
| EC Number | 3.4.21.53 |
| Enzyme Function | FUNCTION: ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner. Regulates mitochondrial DNA (mtDNA) copy number and transcription by stabilizing the mitochondrial TFAM:mtDNA ratio via selective degradation of TFAM. {ECO:0000255|HAMAP-Rule:MF_03120, ECO:0000269|PubMed:20930118}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 564..571; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_03120 |
| Features | Active site (2); Alternative sequence (1); Chain (1); Compositional bias (2); Domain (2); Mutagenesis (1); Nucleotide binding (1); Region (2); Transit peptide (1) |
| Keywords | ATP-binding;Alternative splicing;DNA-binding;Hydrolase;Mitochondrion;Nucleotide-binding;Protease;Reference proteome;Serine protease;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000255|HAMAP-Rule:MF_03120, ECO:0000269|PubMed:20930118}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15834148; 20096722; 20220848; 20371351; 20439780; 21074052; 22758915; 22851652; 23071443; 23087838; 24114784; 24874806; 25122763; 25294943; 26290570; 26370122; 26526100; 26865094; 26870755; 27346357; 27582081; 27768896; 27916526; 27956707; 28017472; 28814717; 30249657; 30260564; 30374414; 30378026; 30518747; 30865561; 30899273; 30943413; 31100073; 31132524; 31722958; 32444642; 32958650; 32984330; 33075064; 33477509; 33827210; |
| Motif | |
| Gene Encoded By | |
| Mass | 115,033 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |