| IED ID | IndEnz0002003860 |
| Enzyme Type ID | protease003860 |
| Protein Name |
Lon protease homolog, mitochondrial EC 3.4.21.53 Fragment |
| Gene Name | PIM1 PICST_64463 |
| Organism | Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Debaryomycetaceae Scheffersomyces Scheffersomyces stipitis (Yeast) (Pichia stipitis) Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) |
| Enzyme Sequence | MLRTSCTSSLRRVVGKYVVSPLVASQIRFATSSVRSQPYLLNSELTELPAQFKRYSSILLTEKPEGDVPESGPEPSGESGISEKSNVENDKHDGNDEIKPEAEKNEKDEIEKPEIDKDAIVETDGVSESSVENVSGSSSAAGGASAPPSGNSNNNNNNNNNNNDNDEPNEIVTNAGTGLYPPLLAIPMKDRPPLPGRPFAINITDPEVIRSIYTIIDKREPYFVLFHVKDPNEGDTDVINSKDSVYNIGVHCQIIRHTTPRPGVFNVLGYPLERCSLADLSTPSEKKGETETRKEGENFPTSYLKGLKVSYATVKPVKDEPFDKTSTDIKSLVESLKALLSKMGAKNPLEKLQIKEGTELVNDPPRFADFVGSTIHGDPKKIQEILESLNIQTRLSKALELLKVELKASLIKENTIHNLSTKADEYQTRLFIKEFIKELQKRAGIVESDDKKTSKFDERLKHLKMTEEALEAYNAEKAKMESQNEHSSELGVSERYLDWLTSIPWGIYSKDRFNIKQAREILDRDHYGLKDVKDRILEFISMGRVSGKVDGKILCLTGPPGTGKTSIAKSIAESLNRKYVRIAMGGIQDVHEVKGHRRTYVGSIPGRIISALKQAKTSNPLMLIDEIDKLDLSRSGGASSAFLEILDPEQNNAFVDNYIDVKVDLSKVLFVCTANYLGNISPPLRDRMEIIEVNGYTNNEKIEIAKRHLIPDAAKKAGLEGGHVVIETKTISRLIEKYCRESGLRNIKKLITRIFSKASLKIVEEVEAREGESKSKSEEAKSEAITGSVTEISVEDATVKAQSIEEPSVESASQKVDEAKPVESEELKSDEEEEEVVKLEIPDDIKLEITSANLKDYVGPEIYTRDRVYDIPPPGVATGLSYSTSGNGDALYIESILTHSIGSGSGHASIHVTGSLKDVMKESASIAYSFAKSYMVKNYPENRFFEAAEIHVHCPDGAIPKDGPSAGISFTSSLISLALQKPLPPTIAMTGEITVTGRVLAVGGLREKILGAKRYGCNTIIFPKDIENELEEIPEEVKEGVKFIPVEWYQDVFDEIFPNLSSDEGNEVWKEEFNKLDKKKASNKKK |
| Enzyme Length | 1086 |
| Uniprot Accession Number | A3M072 |
| Absorption | |
| Active Site | ACT_SITE 965; /evidence=ECO:0000255|HAMAP-Rule:MF_03120; ACT_SITE 1008; /evidence=ECO:0000255|HAMAP-Rule:MF_03120 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins in presence of ATP.; EC=3.4.21.53; Evidence={ECO:0000255|HAMAP-Rule:MF_03120}; |
| DNA Binding | |
| EC Number | 3.4.21.53 |
| Enzyme Function | FUNCTION: ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner. {ECO:0000255|HAMAP-Rule:MF_03120}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 558..565; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_03120 |
| Features | Active site (2); Chain (1); Compositional bias (4); Domain (2); Erroneous initiation (1); Non-terminal residue (1); Nucleotide binding (1); Region (3); Transit peptide (1) |
| Keywords | ATP-binding;DNA-binding;Hydrolase;Mitochondrion;Nucleotide-binding;Protease;Reference proteome;Serine protease;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000255|HAMAP-Rule:MF_03120}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 120,080 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |