| IED ID | IndEnz0002003886 |
| Enzyme Type ID | protease003886 |
| Protein Name |
Mitochondrial-processing peptidase subunit beta EC 3.4.24.64 Beta-MPP |
| Gene Name | mppB DDB_G0288777 |
| Organism | Dictyostelium discoideum (Slime mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Amoebozoa Evosea Eumycetozoa Dictyostelia (dictyostelid cellular slime molds) Dictyosteliales Dictyosteliaceae Dictyostelium Dictyostelium discoideum (Slime mold) |
| Enzyme Sequence | MSNITKLFVKSTKNFSRSFSRKTVDPSYLKISPETKITTLSNGIRVATEQTYGEVASVGVWVDSGSVYETDKNNGVAHFLEHMIFKGTAKRPTPQSIETEIENMGGSLNAFTSREHSAYYMKVLKDNVPNAVDILSDILQNSKFETSLIEQERDTILSENDYIQSKEDEVVFDQLHAAAFQGSALGRTILGPVENIKSITREQIQEFINENYTGDRLVISAAGAVNHEQLVEQVKEKFANVKMSQVSKDVKRAAITNDFIGSELRVRDDEQPLIHFAVAVRALPWTDPDYFVLELIQTMIGNWNRGIAAGKNIASNLGEIVATEDLAESYSTFFTCYQDTGLFGNYGVCQPERVDDLVAEMLKEWQRIATSCNKNEVERNKQKLLATTLMQYDGTSKVCEGIGRQILTLGRRLSPFEVYTRINEITVADVQRVASTLLRDVSPAVTAIGPIANYPDYNFVKGWTYWNRL |
| Enzyme Length | 469 |
| Uniprot Accession Number | Q4W6B5 |
| Absorption | |
| Active Site | ACT_SITE 81; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Activity Regulation | ACTIVITY REGULATION: Binding to alpha subunit is required for catalytic activity. {ECO:0000250|UniProtKB:P10507}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.; EC=3.4.24.64; Evidence={ECO:0000250|UniProtKB:P10507}; |
| DNA Binding | |
| EC Number | 3.4.24.64 |
| Enzyme Function | FUNCTION: Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins (Probable). Preferentially, cleaves after an arginine at position P2 (By similarity). Plays an essential role in mitochondrial biogenesis (PubMed:18603769). {ECO:0000250|UniProtKB:Q03346, ECO:0000269|PubMed:18603769, ECO:0000305|PubMed:18603769}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3) |
| Keywords | Hydrolase;Membrane;Metal-binding;Metalloprotease;Mitochondrion;Mitochondrion inner membrane;Oxidoreductase;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | INDUCTION: Antisense RNA inhibition of the mppB gene induces gene expression of nuclear-encoded mitochondrial proteins (mppA and cxdA) and surprisingly also of the mppB gene itself. These results suggest that antisense RNA inhibition of mppB induces gene expression of mitochondrial proteins, presumably in a retrograde signaling manner. Up-regulated by Pseudomonas aeruginosa, PAO1 strain and PA14 strain infection. {ECO:0000269|PubMed:18590548, ECO:0000269|PubMed:18603769}. |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000269|PubMed:18603769}. Mitochondrion matrix {ECO:0000269|PubMed:18603769}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 19589376; 22120990; |
| Motif | |
| Gene Encoded By | |
| Mass | 52,626 |
| Kinetics | |
| Metal Binding | METAL 78; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 82; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 159; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P10507 |
| Rhea ID | |
| Cross Reference Brenda |