| IED ID |
IndEnz0002003892 |
| Enzyme Type ID |
protease003892 |
| Protein Name |
Response regulator ArlR
|
| Gene Name |
arlR SAOUHSC_01420 |
| Organism |
Staphylococcus aureus (strain NCTC 8325 / PS 47) |
| Taxonomic Lineage |
cellular organisms
Bacteria
Terrabacteria group
Firmicutes
Bacilli
Bacillales
Staphylococcaceae
Staphylococcus
Staphylococcus aureus
Staphylococcus aureus (strain NCTC 8325 / PS 47)
|
| Enzyme Sequence |
MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSHYYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKPFDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLAENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVIR |
| Enzyme Length |
219 |
| Uniprot Accession Number |
Q9KJN4 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
DNA_BIND 122..219; /note=OmpR/PhoB-type; /evidence=ECO:0000255|PROSITE-ProRule:PRU01091 |
| EC Number |
|
| Enzyme Function |
FUNCTION: Member of the two-component regulatory system ArlS/ArlR involved in the regulation of adhesion, autolysis, multidrug resistance and virulence. ArlS/ArlR affects expression of the multidrug resistance transporter NorA and interacts with both Agr (virulence accessory gene regulator) (negatively) and SarA (staphylococcal accessory regulator) (positively) to modulate several virulence factor genes, including ssp (serine protease), spa (surface protein A) and hla (alpha-hemolysin). Could inhibit biofilm development by a mechanism independent of the presence of the poly-N-acetylglucosamine (PNAG). Also, Arl proteins are required for the efficient activity of DNA gyrase inhibitors and high osmolarity on spa expression. {ECO:0000269|PubMed:10633099, ECO:0000269|PubMed:10869073, ECO:0000269|PubMed:11454217, ECO:0000269|PubMed:15528666, ECO:0000269|PubMed:16030226}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Beta strand (6); Chain (1); DNA binding (1); Domain (1); Helix (5); Modified residue (1); Turn (2) |
| Keywords |
3D-structure;Activator;Cytoplasm;DNA-binding;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Two-component regulatory system;Virulence |
| Interact With |
|
| Induction |
INDUCTION: Activated by Agr, SarA, SarV and MgrA. |
| Subcellular Location |
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. |
| Modified Residue |
MOD_RES 52; /note=4-aspartylphosphate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00169 |
| Post Translational Modification |
PTM: Phosphorylated by ArlS. {ECO:0000305}. |
| Signal Peptide |
|
| Structure 3D |
X-ray crystallography (6) |
| Cross Reference PDB |
6IJU;
6IS1;
6IS2;
6IS3;
6IS4;
6KYX;
|
| Mapped Pubmed ID |
31598698;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
25,498 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
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| Cross Reference Brenda |
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