| IED ID | IndEnz0002003897 |
| Enzyme Type ID | protease003897 |
| Protein Name |
DNA methyltransferase CcrM M.CcrMI EC 2.1.1.72 Adenine-specific methyltransferase CcrMI Type II methyltransferase M.CcrNAI M.CcrNAI |
| Gene Name | ccrMIM ccrM CCNA_00382 |
| Organism | Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Alphaproteobacteria Caulobacterales Caulobacteraceae Caulobacter Caulobacter vibrioides (Caulobacter crescentus) Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus) |
| Enzyme Sequence | MKFGPETIIHGDCIEQMNALPEKSVDLIFADPPYNLQLGGDLLRPDNSKVDAVDDHWDQFESFAAYDKFTREWLKAARRVLKDDGAIWVIGSYHNIFRVGVAVQDLGFWILNDIVWRKSNPMPNFKGTRFANAHETLIWASKSQNAKRYTFNYDALKMANDEVQMRSDWTIPLCTGEERIKGADGQKAHPTQKPEALLYRVILSTTKPGDVILDPFFGVGTTGAAAKRLGRKFIGIEREAEYLEHAKARIAKVVPIAPEDLDVMGSKRAEPRVPFGTIVEAGLLSPGDTLYCSKGTHVAKVRPDGSITVGDLSGSIHKIGALVQSAPACNGWTYWHFKTDAGLAPIDVLRAQVRAGMN |
| Enzyme Length | 358 |
| Uniprot Accession Number | B8GZ33 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72; Evidence={ECO:0000305|PubMed:8289276, ECO:0000305|PubMed:8577742}; |
| DNA Binding | |
| EC Number | 2.1.1.72 |
| Enzyme Function | FUNCTION: A beta subtype methylase that recognizes the double-stranded sequence 5'-GANTC-3' and methylates non-modifed A-2 on the hemimethylated, post-replicative DNA (PubMed:12654995) (Probable). Functions only in the predivisional cell. Responsible for 5'-GANTC-3' methylation in the cell; remethylation of hemimethylated sites generated after replication fork passage occurs late in the predivisional cell, near completion of chromosome replication but prior to cell division. Constitutive expression of the methylase leads to morphologically aberrant cells as well as cells that have undergone additional chromosome replication. Contributes to the accurate cell-cycle control of DNA replication and cellular morphology (PubMed:8289276, PubMed:8577742). Opens a bubble in the DNA at the recognition site, allowing precise recognition of the sequence and ensuring enzyme specificity (By similarity). Can fully replace its ortholog in R.meliloti (PubMed:9294447). {ECO:0000250|UniProtKB:P0CAW2, ECO:0000269|PubMed:8289276, ECO:0000269|PubMed:8577742, ECO:0000269|PubMed:9294447, ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:8289276}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Region (3); Sequence conflict (1) |
| Keywords | DNA replication;DNA-binding;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase |
| Interact With | |
| Induction | INDUCTION: Transcribed from 80 minutes in synchronized cells. {ECO:0000269|PubMed:8289276}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Rapidly degraded by Lon protease prior to cell division. {ECO:0000269|PubMed:8666236}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 39,665 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15197 |
| Cross Reference Brenda |