| IED ID | IndEnz0002003940 |
| Enzyme Type ID | protease003940 |
| Protein Name |
Calpain-2 catalytic subunit EC 3.4.22.53 Calcium-activated neutral proteinase 2 CANP 2 Calpain M-type Calpain-2 large subunit Millimolar-calpain M-calpain Fragment |
| Gene Name | CAPN2 |
| Organism | Oryctolagus cuniculus (Rabbit) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Lagomorpha Leporidae (rabbits and hares) Oryctolagus Oryctolagus cuniculus (Rabbit) |
| Enzyme Sequence | QKLIRIRNPWGEVEWTGRWNDNCPNWNTVDPEVRERLAERHEDGEFWMSFSDFLRHYSRLEICNLTPDTLTSDTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYVIKLEEEDEDQEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELRGQTNIHLGKNFFLTTRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKNGDFCVRVFSEKKADYQAVDDEIEADLEEADVSEDDIDDGFRRLFAQLAGEDAEISAFELQNILRRVLAKRQDIKTDGLSIETCKIMVDMLDSDGTGKLGLKEFYVLWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKLPCQLHEVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPDNTGMIQLDLISWLCFSVL |
| Enzyme Length | 422 |
| Uniprot Accession Number | P06814 |
| Absorption | |
| Active Site | ACT_SITE 8; /evidence="ECO:0000255|PROSITE-ProRule:PRU00239, ECO:0000255|PROSITE-ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089, ECO:0000255|PROSITE-ProRule:PRU10090" |
| Activity Regulation | ACTIVITY REGULATION: Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin. |
| Binding Site | |
| Calcium Binding | CA_BIND 307..318; /note=1; /evidence=ECO:0000269|PubMed:3038855; CA_BIND 337..348; /note=2; /evidence=ECO:0000269|PubMed:3038855 |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Broad endopeptidase specificity.; EC=3.4.22.53; |
| DNA Binding | |
| EC Number | 3.4.22.53 |
| Enzyme Function | FUNCTION: Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs. {ECO:0000250|UniProtKB:O08529, ECO:0000250|UniProtKB:P17655}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Calcium binding (2); Chain (1); Domain (4); Metal binding (19); Non-terminal residue (1); Region (3) |
| Keywords | Calcium;Cell membrane;Cytoplasm;Hydrolase;Membrane;Metal-binding;Protease;Reference proteome;Repeat;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 49,494 |
| Kinetics | |
| Metal Binding | METAL 14; /note=Calcium 4; /evidence=ECO:0000250; METAL 21; /note=Calcium 4; /evidence=ECO:0000250; METAL 45; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 264; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 267; /note=Calcium 5; /evidence=ECO:0000250; METAL 269; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 274; /note=Calcium 5; /evidence=ECO:0000250; METAL 307; /note=Calcium 6; /evidence=ECO:0000250; METAL 309; /note=Calcium 6; /evidence=ECO:0000250; METAL 311; /note=Calcium 6; via carbonyl oxygen; /evidence=ECO:0000250; METAL 313; /note=Calcium 6; via carbonyl oxygen; /evidence=ECO:0000250; METAL 318; /note=Calcium 6; /evidence=ECO:0000250; METAL 337; /note=Calcium 7; /evidence=ECO:0000250; METAL 339; /note=Calcium 7; /evidence=ECO:0000250; METAL 341; /note=Calcium 7; via carbonyl oxygen; /evidence=ECO:0000250; METAL 343; /note=Calcium 7; via carbonyl oxygen; /evidence=ECO:0000250; METAL 348; /note=Calcium 7; /evidence=ECO:0000250; METAL 380; /note=Calcium 1; /evidence=ECO:0000250; METAL 383; /note=Calcium 1; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.53; |