Detail Information for IndEnz0002003962
IED ID IndEnz0002003962
Enzyme Type ID protease003962
Protein Name Gag polyprotein
Core polyprotein

Cleaved into: Matrix protein p15
MA
; RNA-binding phosphoprotein p12
pp12
Gene Name gag
Organism Friend spleen focus-forming virus (isolate 502) (FSFFV)
Taxonomic Lineage Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Orthoretrovirinae Gammaretrovirus unclassified Gammaretrovirus Spleen focus-forming virus Friend spleen focus-forming virus (FSFFV) Friend spleen focus-forming virus (isolate 502) (FSFFV)
Enzyme Sequence MGQTVTTPLSLTLEHWEDVQRTASNQSVDVKKRRWVTFCSAEWPTFGVGWPQDGTFNLDIILQVKSKVFSPGPHGHPDQVPYIVTWEAIAYEPPPWVKPFVSPKLSPSPTAPILPSGPSTQPPPRSALYPALTPSIKPGPSPIMADLSLTFSQKTLRRTEDRDRPPLTEMATEKRPPPLLRFLPPLP
Enzyme Length 187
Uniprot Accession Number P03331
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release (By similarity). {ECO:0000250}.; FUNCTION: Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (3); Compositional bias (2); Initiator methionine (1); Lipidation (1); Motif (2); Region (1); Site (1)
Keywords Host cell membrane;Host membrane;Host-virus interaction;Lipoprotein;Membrane;Myristate;RNA-binding;Viral budding;Viral budding via the host ESCRT complexes;Viral matrix protein;Viral release from host cell;Virion
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: [Gag polyprotein]: Virion {ECO:0000250}. Host cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}.; SUBCELLULAR LOCATION: [Matrix protein p15]: Virion {ECO:0000305}.
Modified Residue
Post Translational Modification PTM: Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity). {ECO:0000250}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 109..112; /note=PTAP/PSAP motif; MOTIF 128..132; /note=LYPX(n)L motif
Gene Encoded By
Mass 20,804
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda