| IED ID | IndEnz0002004068 |
| Enzyme Type ID | protease004068 |
| Protein Name |
Ferrochelatase, mitochondrial EC 4.99.1.1 Heme synthase Protoheme ferro-lyase |
| Gene Name | HEM15 YOR176W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MLSRTIRTQGSFLRRSQLTITRSFSVTFNMQNAQKRSPTGIVLMNMGGPSKVEETYDFLYQLFADNDLIPISAKYQKTIAKYIAKFRTPKIEKQYREIGGGSPIRKWSEYQATEVCKILDKTCPETAPHKPYVAFRYAKPLTAETYKQMLKDGVKKAVAFSQYPHFSYSTTGSSINELWRQIKALDSERSISWSVIDRWPTNEGLIKAFSENITKKLQEFPQPVRDKVVLLFSAHSLPMDVVNTGDAYPAEVAATVYNIMQKLKFKNPYRLVWQSQVGPKPWLGAQTAEIAEFLGPKVDGLMFIPIAFTSDHIETLHEIDLGVIGESEYKDKFKRCESLNGNQTFIEGMADLVKSHLQSNQLYSNQLPLDFALGKSNDPVKDLSLVFGNHEST |
| Enzyme Length | 393 |
| Uniprot Accession Number | P16622 |
| Absorption | |
| Active Site | ACT_SITE 351; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 H(+) + heme b = Fe(2+) + protoporphyrin IX; Xref=Rhea:RHEA:22584, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, ChEBI:CHEBI:57306, ChEBI:CHEBI:60344; EC=4.99.1.1; |
| DNA Binding | |
| EC Number | 4.99.1.1 |
| Enzyme Function | FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (12); Chain (1); Helix (19); Sequence conflict (1); Transit peptide (1); Turn (4) |
| Keywords | 3D-structure;Direct protein sequencing;Heme biosynthesis;Iron;Lyase;Membrane;Mitochondrion;Mitochondrion inner membrane;Porphyrin biosynthesis;Reference proteome;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Note=It is bound to the mitochondrial inner membrane in eukaryotic cells with its active site on the matrix side of the membrane. |
| Modified Residue | |
| Post Translational Modification | PTM: The leader peptide may be processed in two proteolytic steps, first between Ser-23 and Phe-24, second and by a different protease, to yield the mature protein. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 1L8X; 1LBQ; |
| Mapped Pubmed ID | 10858295; 11212295; 11283351; 11805837; 11831847; 11939775; 12427010; 12668611; 1304908; 16697062; 16843540; 17352532; 18156292; 18227070; 18593702; 18779372; 19930686; 20008079; 21776189; 24130505; 25028499; 25640729; 25999509; 26354769; 27026703; 27317660; 27387517; 7035824; 7629135; 8473299; 8818224; |
| Motif | |
| Gene Encoded By | |
| Mass | 44,596 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22584 |
| Cross Reference Brenda | 4.99.1.1; |