| IED ID | IndEnz0002004089 |
| Enzyme Type ID | protease004089 |
| Protein Name |
Leucine aminopeptidase 1 EC 3.4.11.- Leucyl aminopeptidase 1 LAP1 |
| Gene Name | lap1 |
| Organism | Aspergillus sojae |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus sojae |
| Enzyme Sequence | MRFLPCIATLAATASALAIGDHIRSDDQYVLELGPGETKVVTEAEKWALRAEGKRFFDITGRTSSLELASNKKQKLAVTYPDSVQHNETVQNLINSLDKKNFETVLQPFSEFHNRYYKSDNGKKSSEWLQGKIQEIISASGAKGVTVEPFKHFFPQSSLIAKIPGKSDKTIVLGAHQDSINLNSPSEGRAPSADDDGSGVVTILEAFRVLLTDEKVAAGEAPNTVEFHFYAGEEGGLLGSQDIFEQYLQKSRDVKAMLQQDMTGYTKGTTDAGKPESIGIITDNVDENLTKFLKIIVDAYCTIPTVDSKCGYGCSDHASATKYGYPAAFAFESAFGDDSPYIHSADDTIETVNFDHVLQHGRLTLGFAYELAFADSL |
| Enzyme Length | 377 |
| Uniprot Accession Number | Q8J2N2 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Activity is strongly inhibited by metalchelating agents, such as EDTA and dipicolinic acid. {ECO:0000269|PubMed:12031491}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.11.- |
| Enzyme Function | FUNCTION: Extracellular aminopeptidase that allows assimilation of proteinaceous substrates. {ECO:0000269|PubMed:12031491}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 70 degrees Celsius. {ECO:0000269|PubMed:12031491}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.1-10. {ECO:0000269|PubMed:12031491}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (1); Glycosylation (2); Metal binding (6); Propeptide (1); Signal peptide (1) |
| Keywords | Aminopeptidase;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Protease;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 41,179 |
| Kinetics | |
| Metal Binding | METAL 176; /note=Zinc 1; /evidence=ECO:0000250; METAL 195; /note=Zinc 1; /evidence=ECO:0000250; METAL 195; /note=Zinc 2; catalytic; /evidence=ECO:0000250; METAL 234; /note=Zinc 2; catalytic; /evidence=ECO:0000250; METAL 261; /note=Zinc 1; /evidence=ECO:0000250; METAL 343; /note=Zinc 2; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.11.1;3.4.11.22; |