| IED ID | IndEnz0002004118 |
| Enzyme Type ID | protease004118 |
| Protein Name |
IgM protease EC 3.4.22.- Immunoglobulin M-degrading enzyme of S.suis IdeSsuis |
| Gene Name | ide SSU0496 |
| Organism | Streptococcus suis (strain P1/7) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus Streptococcus suis Streptococcus suis (strain P1/7) |
| Enzyme Sequence | MNIQERFSLRKSAVGLVSVSLLCAIYTSTVAADTVVTGVNEIIEESQVKDEVSIESEKNESLDGSNIEIVEEIADNIPSPVIAEGEVAVEMKVDRGTENVVSRNDTEVTTSEQNQIEVTETKEILNQTSYQTESGEQRQIIWAHGITPPAMEQSGGFVKEKYGDYLNYTAPFEAGKGYYDTNKSLNASFIDLNLCFAAVSSNMVHWWLEQNSSYVERYLKEKKGTVNVEENYAITDLRRYINSFQNQQNSRVFDMFKTYYGYRTNGFVSDALVDLFINGYKPKAQGGVNLEDSQLVPDSRGGFFYDVFKEKKLTNRIFSGSYERFGEDVRTVLESKGLLGLTYRTLGYATHIVTVWGAEYDNQGKIKAVYITDSDDQQEQIGLKRMGITRDASGNPRLNNHMKNNSAGALLDYVHTIRLGQDLWEEYFNPLAKAKETASQTLADTKKALDLSIQGQSELPESMRLIYLEKLNNLYNQGILSIQKAESSEMLSGALENGLNSLKSLDFPISEVGNALAPDLPVGDRSTVSDVDSLSSQETSSTNLEADTENAGIIADGTNQLHFPVEAQTTSSVEAEGDNVFEQEADTLPIIIENKDEFGSELSRNMQTSETDSLVVAVEEDVKNDEVAQVEELLESEKVENQSSELLSDTLIVESANDKEEDRVEAVVSEQPDSIPHQNVEISLVEPTNVETETVVTPINDAATPHGSPTYIDNSVTESVATPLEKDSIQAGETEIAEPTSSESTNVETETVVTPVNDVATPHGSPTYIDNSVTESVATPLEKDSIQAGETEIAEPTSSESTNVETETVVTPVNDVATPHGSPTYIDNSVTESVATPLEKDSIQAGETEIAEPTSSESTSVEAELVDNSEIHAATSSVTPCGSSAYADGSTTESVATPLEKDSIQTGNTEIAEPTSSKSTNVEAASVDNSEIHADASLTAVSSVNLDNPVIEPVAISLIGSKRDTNAEVEVSSLSKREVRKTNTDGLISVQSKVIKKELLESSLAEAGSPLLEATIAQSSNSNSTEIGMSYQNTVLLESNNTERQVSKAEIVMEHKETELVETVSSASEPVVLVENISQTSNNTIESGKNMGVQSQAGAKQILGVEQSSKVSTPTSRQIMGVGLLTLVLGSALGLLKKRRK |
| Enzyme Length | 1141 |
| Uniprot Accession Number | C5W022 |
| Absorption | |
| Active Site | ACT_SITE 195; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | ACTIVITY REGULATION: IgM cleavage is inhibited by iodoacetamide but not by AEBSF, bestatin, E-64, Z-LVG-CHN(2), or EDTA. {ECO:0000269|PubMed:23243300}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Catalyzes the specific cleavage of porcine IgM bound to the bacterial surface. Can degrade only IgM but neither IgG nor IgA, and is host specific, as it exclusively cleaves porcine IgM but not IgM from six other species, including human, mouse and a closely related member of the Suidae family. Promotes survival in porcine blood. Is thus involved in a so-far-unknown mechanism of host-pathogen interaction at an early stage of the host immune response. {ECO:0000269|PubMed:23243300}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (3); Region (4); Signal peptide (1); Transmembrane (1) |
| Keywords | Cell membrane;Hydrolase;Membrane;Protease;Secreted;Signal;Thiol protease;Transmembrane;Transmembrane helix;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23243300}; Single-pass membrane protein {ECO:0000269|PubMed:23243300}. Secreted {ECO:0000269|PubMed:23243300}. Note=Is released into the extracellular space and remains at least transiently attached to the bacterial surface, likely via its C-terminal transmembrane domain. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..32; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 123,714 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |