| IED ID | IndEnz0002004145 |
| Enzyme Type ID | protease004145 |
| Protein Name |
Prostate-specific antigen PSA EC 3.4.21.77 Gamma-seminoprotein Seminin Kallikrein-3 P-30 antigen Semenogelase |
| Gene Name | KLK3 APS |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP |
| Enzyme Length | 261 |
| Uniprot Accession Number | P07288 |
| Absorption | |
| Active Site | ACT_SITE 65; /note=Charge relay system; /evidence=ECO:0000269|PubMed:18187150; ACT_SITE 120; /note=Charge relay system; /evidence=ECO:0000269|PubMed:18187150; ACT_SITE 213; /note=Charge relay system; /evidence=ECO:0000269|PubMed:18187150 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. {ECO:0000269|PubMed:1725227, ECO:0000269|PubMed:18187150}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: -Tyr-|-Xaa-.; EC=3.4.21.77; |
| DNA Binding | |
| EC Number | 3.4.21.77 |
| Enzyme Function | FUNCTION: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (5); Beta strand (16); Chain (1); Disulfide bond (5); Domain (1); Erroneous initiation (1); Glycosylation (1); Helix (5); Natural variant (3); Propeptide (1); Sequence caution (1); Sequence conflict (6); Signal peptide (1) |
| Keywords | 3D-structure;Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000269|PubMed:15340161 |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 2ZCH; 2ZCK; 2ZCL; 3QUM; |
| Mapped Pubmed ID | 10218588; 11027412; 11185746; 11751371; 12111704; 12168876; 12376473; 12379487; 12466191; 12865450; 1383255; 15017213; 15174051; 15893813; 16424004; 17047378; 17094454; 18066052; 18264097; 18535354; 19345326; 22019475; 22037582; 22806587; 25241761; 7525634; 7525824; 7538844; 8683730; 8753751; 8817670; |
| Motif | |
| Gene Encoded By | |
| Mass | 28,741 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.77; |