| IED ID | IndEnz0002004158 |
| Enzyme Type ID | protease004158 |
| Protein Name |
Inactive metacaspase-4 TbMCA4 |
| Gene Name | MCA4 |
| Organism | Trypanosoma brucei brucei |
| Taxonomic Lineage | cellular organisms Eukaryota Discoba Euglenozoa Kinetoplastea (kinetoplasts) Metakinetoplastina Trypanosomatida Trypanosomatidae Trypanosoma Trypanozoon Trypanosoma brucei Trypanosoma brucei brucei |
| Enzyme Sequence | MGGCVSTALKVGAETVAEGHIDLISFAINYFKNAVPYIVKYLGRQQRPKEVDMEATLTEAKESKGFQPWKISCQPKGAVRGLFIGVNYGNTEAQLSGCCHDIMMMIGALQKRNFPLTEVVILADEEDVPGRTGEPTRANILRYLAWLAQDAQPNDVLFFHYSGHGTRANARDDDCEEYDQCIVPMDYVENGCIVDNEIHEILVSQLPKGVRLTAVFDCSHSGSMLDLPYAYVCDSSKDGSGSCGMKRVREDNDVQADVLMISACADDEAALGVDNTQDFYESGKDSGGAATFCLTAMMMREEPLTFLDLLVHTREMLKSRGFTQVPHLSASKPINLMQRFSLEGLFPQERTLL |
| Enzyme Length | 353 |
| Uniprot Accession Number | Q8T8E5 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Inactive metacaspase which plays a role in parasite bloodstream form growth and in parasite virulence within the mammalian host. {ECO:0000269|PubMed:21949125}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Initiator methionine (1); Lipidation (1); Mutagenesis (2); Site (1) |
| Keywords | Cell membrane;Cell projection;Cilium;Flagellum;Lipoprotein;Membrane;Myristate;Palmitate;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane {ECO:0000269|PubMed:21949125}; Lipid-anchor {ECO:0000269|PubMed:21949125}; Cytoplasmic side {ECO:0000269|PubMed:21949125}. Secreted {ECO:0000269|PubMed:21949125}. Note=Secreted by the parasite bloodstream form (PubMed:21949125). Membrane localization is required for MCA4 processing but is dispensable for MCA4 secretion, and parasite growth and virulence in the mammalian host (PubMed:21949125). {ECO:0000269|PubMed:21949125}. |
| Modified Residue | |
| Post Translational Modification | PTM: Palmitoylated. {ECO:0000269|PubMed:21949125}.; PTM: Proteolytic cleavage by MCA3 occurs prior or during secretion and requires MCA4 membrane localization (PubMed:21949125). Cleavage is dispensable for secretion and parasite growth and virulence in the mammalian host (PubMed:21949125). In vitro, can be cleaved by MCA2 but specifically cleaved by MCA3 in vivo (PubMed:21949125). {ECO:0000269|PubMed:21949125}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 38,993 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |