| IED ID | IndEnz0002004218 |
| Enzyme Type ID | protease004218 |
| Protein Name |
Histone H2B type 1-H H2B-clustered histone 9 Histone H2B.j H2B/j |
| Gene Name | H2BC9 H2BFJ HIST1H2BH |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MPDPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK |
| Enzyme Length | 126 |
| Uniprot Accession Number | Q93079 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Cross-link (4); Glycosylation (1); Initiator methionine (1); Modified residue (73); Region (1) |
| Keywords | Acetylation;Chromosome;DNA-binding;Direct protein sequencing;Glycoprotein;Hydroxylation;Isopeptide bond;Methylation;Nucleosome core;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation |
| Interact With | P54253; P42858; Q05BL1 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus. Chromosome. |
| Modified Residue | MOD_RES 2; /note="N-acetylproline"; /evidence="ECO:0000250|UniProtKB:P23527"; MOD_RES 6; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 6; /note="N6-(beta-hydroxybutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:27105115"; MOD_RES 6; /note="N6-acetyllysine; alternate"; /evidence="ECO:0000269|PubMed:16283522, ECO:0000269|PubMed:16627869"; MOD_RES 6; /note="N6-butyryllysine; alternate"; /evidence="ECO:0000269|PubMed:27105113"; MOD_RES 6; /note="N6-crotonyllysine; alternate"; /evidence="ECO:0000269|PubMed:21925322"; MOD_RES 6; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 12; /note="N6-(beta-hydroxybutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:27105115"; MOD_RES 12; /note="N6-acetyllysine; alternate"; /evidence="ECO:0000269|PubMed:16627869"; MOD_RES 12; /note="N6-crotonyllysine; alternate"; /evidence="ECO:0000269|PubMed:21925322"; MOD_RES 12; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 13; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 13; /note="N6-acetyllysine; alternate"; /evidence="ECO:0000269|PubMed:16283522, ECO:0000269|PubMed:16627869"; MOD_RES 13; /note="N6-crotonyllysine; alternate"; /evidence="ECO:0000269|PubMed:21925322"; MOD_RES 15; /note="Phosphoserine; by STK4/MST1"; /evidence="ECO:0000269|PubMed:12757711"; MOD_RES 16; /note="N6-acetyllysine; alternate"; /evidence="ECO:0000269|PubMed:16283522, ECO:0000269|PubMed:16627869"; MOD_RES 16; /note="N6-crotonyllysine; alternate"; /evidence="ECO:0000269|PubMed:21925322"; MOD_RES 16; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 17; /note="N6-(beta-hydroxybutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:27105115"; MOD_RES 17; /note="N6-acetyllysine; alternate"; /evidence="ECO:0000269|PubMed:16627869"; MOD_RES 17; /note="N6-crotonyllysine; alternate"; /evidence="ECO:0000269|PubMed:21925322"; MOD_RES 17; /note="N6-glutaryllysine; alternate"; /evidence="ECO:0000269|PubMed:31542297"; MOD_RES 17; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 21; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 21; /note="N6-(beta-hydroxybutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:27105115"; MOD_RES 21; /note="N6-acetyllysine; alternate"; /evidence="ECO:0000269|PubMed:16283522, ECO:0000269|PubMed:16627869"; MOD_RES 21; /note="N6-butyryllysine; alternate"; /evidence="ECO:0000269|PubMed:27105113"; MOD_RES 21; /note="N6-crotonyllysine; alternate"; /evidence="ECO:0000269|PubMed:21925322"; MOD_RES 21; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 24; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 24; /note="N6-acetyllysine; alternate"; /evidence="ECO:0000250|UniProtKB:P33778"; MOD_RES 24; /note="N6-crotonyllysine; alternate"; /evidence="ECO:0000269|PubMed:21925322"; MOD_RES 24; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 25; /note="N6-(2-hydroxyisobutyryl)lysine"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 35; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 35; /note="N6-(beta-hydroxybutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:27105115"; MOD_RES 35; /note="N6-crotonyllysine; alternate"; /evidence="ECO:0000269|PubMed:21925322"; MOD_RES 35; /note="N6-glutaryllysine; alternate"; /evidence="ECO:0000269|PubMed:31542297"; MOD_RES 35; /note="N6-succinyllysine; alternate"; /evidence="ECO:0000269|PubMed:22389435"; MOD_RES 37; /note="Phosphoserine; by AMPK"; /evidence="ECO:0000250|UniProtKB:Q64478"; MOD_RES 44; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 44; /note="N6-glutaryllysine; alternate"; /evidence="ECO:0000269|PubMed:31542297"; MOD_RES 44; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 47; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 47; /note="N6-glutaryllysine; alternate"; /evidence="ECO:0000269|PubMed:31542297"; MOD_RES 47; /note="N6-methyllysine; alternate"; /evidence="ECO:0000269|PubMed:16627869"; MOD_RES 58; /note="N6,N6-dimethyllysine; alternate"; /evidence="ECO:0000269|PubMed:16627869"; MOD_RES 58; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 80; /note="Dimethylated arginine"; /evidence="ECO:0000250|UniProtKB:Q96A08"; MOD_RES 86; /note="N6,N6,N6-trimethyllysine; alternate"; /evidence="ECO:0000250|UniProtKB:Q96A08"; MOD_RES 86; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 86; /note="N6-(beta-hydroxybutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:27105115"; MOD_RES 86; /note="N6-acetyllysine; alternate"; /evidence="ECO:0000250|UniProtKB:Q96A08"; MOD_RES 86; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 87; /note="Omega-N-methylarginine"; /evidence="ECO:0000250|UniProtKB:Q96A08"; MOD_RES 93; /note="Omega-N-methylarginine"; /evidence="ECO:0000250|UniProtKB:Q96A08"; MOD_RES 109; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 109; /note="N6-glutaryllysine; alternate"; /evidence="ECO:0000269|PubMed:31542297"; MOD_RES 109; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 109; /note="N6-methyllysine; alternate"; /evidence="ECO:0000269|PubMed:16627869"; MOD_RES 116; /note="Phosphothreonine"; /evidence="ECO:0000250|UniProtKB:Q00729"; MOD_RES 117; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 117; /note="N6-(beta-hydroxybutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:27105115"; MOD_RES 117; /note="N6-glutaryllysine; alternate"; /evidence="ECO:0000269|PubMed:31542297"; MOD_RES 117; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 117; /note="N6-malonyllysine; alternate"; /evidence="ECO:0000269|PubMed:22389435"; MOD_RES 117; /note="N6-methylated lysine; alternate"; /evidence="ECO:0000250|UniProtKB:Q00729"; MOD_RES 117; /note="N6-succinyllysine; alternate"; /evidence="ECO:0000269|PubMed:22389435"; MOD_RES 121; /note="N6-(2-hydroxyisobutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:24681537"; MOD_RES 121; /note="N6-(beta-hydroxybutyryl)lysine; alternate"; /evidence="ECO:0000269|PubMed:27105115"; MOD_RES 121; /note="N6-glutaryllysine; alternate"; /evidence="ECO:0000269|PubMed:31542297"; MOD_RES 121; /note="N6-lactoyllysine; alternate"; /evidence="ECO:0000269|PubMed:31645732"; MOD_RES 121; /note="N6-succinyllysine; alternate"; /evidence="ECO:0000269|PubMed:22389435" |
| Post Translational Modification | PTM: Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons (PubMed:16627869). Monoubiquitinated by DTX3L upon encephalomyocarditis virus (EMCV)-mediated infection (PubMed:26479788). {ECO:0000269|PubMed:16627869, ECO:0000269|PubMed:26479788}.; PTM: Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. {ECO:0000250|UniProtKB:Q64475, ECO:0000269|PubMed:12757711}.; PTM: GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity). {ECO:0000250|UniProtKB:P62807}.; PTM: Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. {ECO:0000269|PubMed:21925322}.; PTM: Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription. {ECO:0000269|PubMed:31645732}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10029069; 10072381; 10318934; 10373014; 10373562; 10438626; 10444591; 10449787; 10471499; 10500157; 10525529; 10550055; 10551868; 10555141; 10562567; 10567546; 10579975; 10652259; 10654609; 10669743; 10679930; 10724175; 10728666; 10734115; 10748112; 10777502; 10809667; 10821277; 10866324; 10904264; 10926844; 10940626; 10959836; 10973490; 11025670; 11103799; 11114888; 11171374; 11178747; 11209945; 11254356; 11278854; 11336508; 11410592; 11493525; 11493912; 11509652; 11511348; 11544178; 11566863; 11571274; 11583633; 11604075; 11673449; 11731253; 11739383; 11784849; 11821381; 11823429; 11891985; 11893489; 11909966; 11934988; 11950880; 12032298; 12086618; 12086873; 12198165; 12208845; 12351676; 12364621; 12368916; 12385756; 12435631; 12447390; 12481029; 12482716; 12483298; 12576332; 12577067; 12591602; 12607003; 12607005; 12697768; 12724404; 12725064; 12736215; 12773400; 12792649; 12798298; 12815623; 12947387; 12954718; 1352886; 1360810; 1363091; 1423634; 14610093; 14695187; 14969726; 14980219; 15033486; 15099518; 15105426; 15158084; 15164066; 15201865; 15262888; 15279782; 15282324; 15304223; 15340071; 15345050; 15468306; 15469825; 15509558; 15514030; 15563468; 15574327; 15580272; 15589157; 15634700; 15654074; 15671018; 15723659; 15750587; 15767661; 15799776; 1582411; 15861382; 15986123; 15998665; 16009131; 16026162; 16061184; 16079285; 16079795; 16085498; 16087863; 16113099; 16123081; 16150728; 16166375; 16166636; 16274244; 16292313; 1630819; 16357870; 16373483; 16377563; 16427009; 16431907; 16500889; 16510874; 16549775; 16603238; 16622420; 16628190; 16651405; 1673098; 16735507; 16756492; 16757478; 16766662; 1682218; 16829525; 1693558; 16936726; 16980581; 16999741; 17010333; 17013392; 17085592; 17113575; 17185360; 17199038; 17210787; 17277772; 17312021; 17332741; 17344414; 17344431; 17355984; 17428788; 17452644; 17463000; 17510434; 17512414; 17525233; 17525332; 17525340; 17541404; 17543860; 17553757; 17637753; 17660710; 17663992; 17666587; 17673620; 17687327; 17707230; 17713477; 17713478; 17761849; 17851529; 17875646; 17977839; 17980597; 17981954; 17994007; 18001824; 18001825; 18006705; 18066052; 18077395; 18164278; 18187655; 18219272; 18223688; 18227514; 18256232; 18256600; 18283110; 18302924; 18316480; 18346456; 18358809; 18367646; 18400894; 18417536; 18485871; 18533032; 18535008; 18552846; 18663141; 18772888; 18772889; 18772891; 18787068; 18832071; 18852463; 18923072; 18945682; 18974356; 19001859; 19020303; 19026781; 19092802; 19098913; 19104046; 19114653; 19115252; 19146767; 19176521; 19203578; 19203579; 19232136; 19234442; 19261749; 19351884; 19398759; 19410543; 19410544; 19410545; 19451217; 19451218; 19462008; 19472184; 19477923; 19509293; 19531582; 19571010; 19575011; 19575673; 19682613; 19703992; 1972280; 19747919; 1975088; 19782034; 19834512; 19883641; 20016603; 20018712; 20023648; 20026581; 20062530; 20075857; 20080577; 20085832; 20123894; 20133701; 20171203; 20211137; 20219941; 20223770; 20231276; 20498280; 20525793; 20566683; 20622854; 20630873; 20651253; 20682797; 20693536; 20836077; 20838592; 20841325; 20937877; 20952535; 20966048; 20974918; 21087926; 21151121; 21151129; 21163962; 21170310; 21282113; 21293379; 21320481; 21324897; 21329877; 21378119; 21427083; 21445275; 21451113; 21489993; 21497760; 21498573; 21508930; 21566127; 21589992; 21606950; 21618588; 21706000; 21768289; 21777816; 21808299; 21808300; 21835891; 21878914; 21926161; 21934649; 21940756; 21979949; 22012064; 22021426; 22056668; 22064703; 22064859; 22110697; 22178396; 22179609; 22234877; 22265404; 22323599; 22323818; 22325352; 22348533; 22373579; 22398447; 22445173; 22466170; 22508508; 22569366; 22570494; 22589545; 22635276; 22661229; 22661230; 22684280; 22704330; 22705304; 22722852; 22751019; 22761581; 22777353; 22810585; 22872084; 22879880; 22908299; 22961379; 22980979; 23022729; 23145106; 23161542; 23172872; 23187890; 23192763; 2330041; 23306437; 23326524; 23333306; 2342578; 23431328; 23435229; 23463006; 23534758; 23555303; 23630040; 23638021; 23663978; 23667505; 23679895; 23723241; 23723417; 23727112; 23752590; 23795651; 23897426; 2392154; 23926102; 23955559; 24003239; 24074865; 24086155; 24174538; 24253042; 24312487; 24368767; 24371278; 24388755; 2444477; 2452725; 24586203; 24667604; 24705354; 24821725; 24854081; 24973012; 25101834; 25135475; 25170678; 25313066; 25512557; 25512564; 25542856; 25593184; 25689043; 2570724; 2571087; 2571936; 25784553; 25913784; 26055325; 26572645; 27062924; 27501258; 27569044; 27798111; 28404884; 28415052; 28854357; 28871079; 2891503; 2898782; 30360396; 3045126; 30761409; 30993897; 3210246; 33801762; 3675579; 3677090; 6577443; 7488013; 7501971; 7559524; 7566114; 7587590; 7710686; 7743939; 7831296; 7831297; 7901228; 7908827; 7914354; 7916164; 8033412; 8093325; 8093858; 8100763; 8650194; 8674428; 8703472; 8710855; 8759021; 8794855; 8843195; 8895582; 8929543; 8999919; 9008167; 9053316; 9106661; 9135082; 9144291; 9150135; 9154799; 9218805; 9242495; 9272954; 9311981; 9347914; 9360992; 9398850; 9443891; 9450758; 9463349; 9463359; 9482740; 9488723; 9501100; 9512523; 9570764; 9651585; 9660953; 9660954; 9662389; 9671459; 9697850; 9735362; 9789037; 9790534; 9826763; 9880483; 9895323; |
| Motif | |
| Gene Encoded By | |
| Mass | 13,892 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |