| IED ID | IndEnz0002004220 |
| Enzyme Type ID | protease004220 |
| Protein Name |
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase stabilizing protein GtfB Glycosyltransferase chaperone Gtf2 Glycosyltransferase stabilizing protein GtfB Glycosyltransferase-stabilizing protein Gtf2 |
| Gene Name | gtfB gtf2 |
| Organism | Streptococcus parasanguinis |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus Streptococcus parasanguinis |
| Enzyme Sequence | MIRLFEWLTQESLDLHYSLEESGIHGTSIVLNDDGFLPEGIISPYTFFCEVEMDGSPLYFNQLEVPYLWQITGTNIEGEIWNRSSKRGVIHYHEPKYLRFVQSVDWLYPDGSIYMTDHYNKYGWAFARTYFFSDQQVSHKKYYTKSGQEVLSENILTGDILLNWKGKVYHFTKKVDFFLFYFKKSGLDLSSIWYNSLGMPFLISYYLGGEGRDILFWQENLADQLPGNMQIIFSGRTSRTKKVIVQDRSVYKKLLHLVEEKNKEMISFLNIIYPKLRENYSRKEILIVTNSDQIEGIETLTDNLSAYTFHIGALTSMSDKLQNIGQKENVLLYPNMSPKTMLDLLEQCDIYLDINHGNEVLSIVRLAFERSLLILAYDNTVHSPIFHHESGIFNHSKPQTLSDWLLNLDDYSQTVSCWRSDLFPMTYRDYKQVLVSNVD |
| Enzyme Length | 439 |
| Uniprot Accession Number | A1C3M0 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Required for the polymorphic O-glycosylation of the serine-rich repeat protein Fap1. A stabilizing protein that is part of the accessory SecA2/SecY2 system specifically required to export Fap1, a serine-rich fimbrial adhesin encoded upstream in the same operon. The GtfA-GtfB (Gtf1-Gtf2 in this bacteria) complex adds GlcNAc from UDP-GlcNAc to Fap1, attaching the first sugar residue. Cannot use not UDP-Glc as substrate. Stabilizes the glycosylation activity of GtfA, causing it to partially localize to the cellular membrane where it is more protease resistant. {ECO:0000269|PubMed:18083807, ECO:0000269|PubMed:20971868, ECO:0000269|PubMed:21862581}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Protein modification; protein glycosylation. {ECO:0000255|HAMAP-Rule:MF_01473, ECO:0000269|PubMed:18083807, ECO:0000269|PubMed:20971868, ECO:0000269|PubMed:21862581}. |
| nucleotide Binding | |
| Features | Chain (1); Mutagenesis (6) |
| Keywords | Cell membrane;Direct protein sequencing;Membrane |
| Interact With | A1C3L9 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:21862581}; Peripheral membrane protein {ECO:0000305|PubMed:21862581}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 51,363 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |