| IED ID | IndEnz0002004248 |
| Enzyme Type ID | protease004248 |
| Protein Name |
Helix-loop-helix protein hlh-2 |
| Gene Name | hlh-2 M05B5.5 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MADPNSQLTSATTVATAAIAQPQVMLPNAYDYPYNIDPTTIQMPDYWSGYHLNPYPPMQTTDIDYSSAFLPTHPPTETPASVAAPTSATSDIKPIHATSSTSTTAPSTAPAPTSTTDVLELKPTTAPATNSAETSAIVAPQPLTNLTAPIDAMSSMYTWPQTYPGYLPPSEDNKASEAVNPYISIPPTYTFGADPSVADFSSYQQQLAGQPNGLGGDTNLVDYNHQFPPAGMSPHFDPNGYPGMTGMPPGSSASSVRNDKSASRATSRRRVQGPPSSGIPTRHSSSSRLSDNESMSDDKDTDRRSQNNARERVRVRDINSAFKELGRMCTQHNQNTERNQTKLGILHNAVSVITQLEEQVRQRNMNPKVMAGMKRKPDDDKMKMLDDNAPSAQFGHPRF |
| Enzyme Length | 399 |
| Uniprot Accession Number | G5EEG9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Transcription factor which binds the E box motif 5'-CA[TC][AG]TG-3' (PubMed:19632181, PubMed:11076762, PubMed:14701877). Plays a key role in the anchor cell/ventral uterine precursor cell (AC/VU) decision; required for VU fate (PubMed:14701877, PubMed:31402303). Regulates expression of lin-12/Notch receptor and putative ligand lag-2 in the presumptive AC and presumptive VU cells (PubMed:31402303). Modulates expression of lag-2 in the gonadal distal tip cells (DTCs) (PubMed:14701877, PubMed:19376107). Involved in formation of the polarised cell membrane of the AC and thus facilitates invasion across the gonadal basement membrane, acting via transcriptional modulation of multiple genes (PubMed:21784067). Involved in specification of the hermaphrodite DTC and the male linker cell, perhaps acting in concert with the homeobox protein, ceh-22 (PubMed:19376107). Plays a role in regulation of migration of DTCs and the modulation of expression of alpha integrin ina-1 and ADAMTS protease gon-1 (PubMed:25982859, PubMed:17588558). Required for DTC maintenance, and for function of the DTC as a niche for germline stem cells (PubMed:19376107). Plays a role in cell-autonomously establishing a neuronal left-right asymmetry (PubMed:21041366). Required for specification of cell fate, acting in concert with lin-32, in the development of the male-specific genital sensilla (simple sense organs) known as rays (PubMed:11076762). Negatively modulates lifespan, perhaps acting by regulating expression of arginine kinases, which in turn results in altered metabolism and homeostasis of reactive oxygen species (ROS) (PubMed:32203922). {ECO:0000269|PubMed:11076762, ECO:0000269|PubMed:14701877, ECO:0000269|PubMed:17588558, ECO:0000269|PubMed:19376107, ECO:0000269|PubMed:19632181, ECO:0000269|PubMed:21041366, ECO:0000269|PubMed:21784067, ECO:0000269|PubMed:25982859, ECO:0000269|PubMed:31402303, ECO:0000269|PubMed:32203922}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (3); Domain (1); Mutagenesis (2); Region (5); Sequence conflict (6) |
| Keywords | DNA-binding;Developmental protein;Neurogenesis;Nucleus;Reference proteome;Transcription;Transcription regulation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11076762}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10778742; 11178279; 11483502; 11799068; 12529635; 12874127; 12952888; 14627726; 14660442; 15282161; 15791247; 16480708; 17090602; 17096596; 17215301; 17369030; 17417969; 18692475; 19386265; 20691175; 21177967; 22267497; 22560298; 22991463; 23791784; 23800452; 24884423; 25487147; 26063786; 26073017; 26159995; 26430702; 28238659; 28602651; 29301976; 29672507; 6593563; 9187144; 9716413; |
| Motif | |
| Gene Encoded By | |
| Mass | 43,193 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |